一个铁硫集群作为一个新的金属中心在一个flavodiiron蛋白质
Maria C Martins1, Célia M Silveira1, Miguel Teixeira1
1Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras, Portugal.
Protein science : a publication of the Protein Society
|June 23, 2025
概括
Syntrophomonas wolfei Class E 类黄铁蛋白 (FDP) 具有一种新的 [3Fe-4S] 铁硫,使用 EPR 和共振拉曼光谱识别. 这一发现揭示了FDP中的新氧化还原中心,可能揭示了新的电子转移途径.
科学领域:
- 生物化学 生物化学
- 微生物学 微生物学
- 频谱学是一种光谱学.
背景情况:
- Syntrophomonas wolfei含有两个H类和E类的黄铁蛋白 (FDP),对于保护无氧细菌免受氧气暴露至关重要.
- 以前预测E类FDP拥有额外的C端域,具有潜在的铁硫中心,与典型的FDP不同.
研究的目的:
- 详细描述S. wolfei的E类FDP,重点关注其独特的C端域.
- 确定铁硫集群的精确性质和flavin辅因子的活性减少状态.
主要方法:
- 野生类型,局部定向突变和S. wolfeiE类FDP的截断变体的酶表征.
- 电子磁共振 (EPR) 和共振拉曼光谱仪用于分析铁硫中心.
- 预测alphafold2结构以评估域同质性.
- 弗拉单核酸 (FMN) 辅因子的光谱分析.
主要成果:
- 在E类FDP的C端域内明确识别了一个 [3Fe-4S]1+/0集群,这是这个酶类的新发现.
- 结构预测揭示了铁硫域与已知的 [3Fe-4S] 1+/0 铁毒素之间的相似性.
- 谱学数据表明,flavin mononucleotide的半子形式是活性减少状态.
- 检测到一种具有独特的紫外线可见光谱的轻微flavin相关物种.
结论:
- 来自S. wolfei的E类FDP含有以前未被识别的 [3Fe-4S] 1+/0 的氧化还原中心.
- 这一发现表明在flavodiiron蛋白中存在潜在的新型电子转移机制.
- 这项研究提供了对弗拉辅因子的氧化还原状态的见解,并确定了一种新的光谱特征.
相关概念视频
02:51Metal-Ligand Bonds
21.5K
The hemoglobin in the blood, the chlorophyll in green plants, vitamin B-12, and the catalyst used in the manufacture of polyethylene all contain coordination compounds. Ions of the metals, especially the transition metals, are likely to form complexes.
In these complexes, transition metals form coordinate covalent bonds, a kind of Lewis acid-base interaction in which both of the electrons in the bond are contributed by a donor (Lewis base) to an electron acceptor (Lewis acid). The Lewis acid in...
In these complexes, transition metals form coordinate covalent bonds, a kind of Lewis acid-base interaction in which both of the electrons in the bond are contributed by a donor (Lewis base) to an electron acceptor (Lewis acid). The Lewis acid in...
21.5K
03:02Colors and Magnetism
12.4K
Color in Coordination Complexes
When atoms or molecules absorb light at the proper frequency, their electrons are excited to higher-energy orbitals. For many main group atoms and molecules, the absorbed photons are in the ultraviolet range of the electromagnetic spectrum, which cannot be detected by the human eye. For coordination compounds, the energy difference between the d orbitals often allows photons in the visible range to be absorbed and emitted, which is seen as colors by the human...
When atoms or molecules absorb light at the proper frequency, their electrons are excited to higher-energy orbitals. For many main group atoms and molecules, the absorbed photons are in the ultraviolet range of the electromagnetic spectrum, which cannot be detected by the human eye. For coordination compounds, the energy difference between the d orbitals often allows photons in the visible range to be absorbed and emitted, which is seen as colors by the human...
12.4K
03:45Formation of Complex Ions
24.0K
A type of Lewis acid-base chemistry involves the formation of a complex ion (or a coordination complex) comprising a central atom, typically a transition metal cation, surrounded by ions or molecules called ligands. These ligands can be neutral molecules like H2O or NH3, or ions such as CN− or OH−. Often, the ligands act as Lewis bases, donating a pair of electrons to the central atom. These types of Lewis acid-base reactions are examples of a broad subdiscipline called coordination...
24.0K
01:43Electron Transport Chain: Complex III and IV
8.2K
During the electron transport chain, electrons from NADH and FADH2 are first transferred to complexes I and II, respectively. These two complexes then transfer the electrons to ubiquinol, which carries them further to complex III. Complex III passes the electrons across the intermembrane space to Cyt c, which carries them further to complex IV. Complex IV donates electrons to oxygen and reduces it to water. As electrons pass through complexes I, III, and IV, the energy released aids the pumping...
8.2K
01:46Electron Transport Chain: Complex I and II
15.1K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
ROS generation is regulated and maintained at moderate levels necessary...
15.1K
02:42Valence Bond Theory
9.7K
Coordination compounds and complexes exhibit different colors, geometries, and magnetic behavior, depending on the metal atom/ion and ligands from which they are composed. In an attempt to explain the bonding and structure of coordination complexes, Linus Pauling proposed the valence bond theory, or VBT, using the concepts of hybridization and the overlapping of the atomic orbitals. According to VBT, the central metal atom or ion (Lewis acid) hybridizes to provide empty orbitals of suitable...
9.7K