在伤口诱导的重编程中,F-Actin极化和微管完整性直接再生模式和细胞外生长的极性
Yi-Ting Huang1, Yun-Ching Yen1, Joop E M Vermeer2
1Graduate Institute of Biochemistry, National Chung Hsing University, Taichung, ROC, Taiwan.
Plant, cell & environment
|June 23, 2025
概括
植物再生依赖于受伤后的细胞重编程. 这项研究揭示了细胞骨网络,特别是活性丝,如何指导这个过程,影响细胞分裂和外生长极性,以有效修复组织.
科学领域:
- 植物生物学 植物生物学
- 细胞力学 细胞力学
- 再生生物学 再生生物学
背景情况:
- 植物具有显著的再生能力来修复损坏.
- 细胞机械感知和重编程是再生的关键,但它们之间的联系尚不清楚.
- 细胞骨网络在伤口诱导再生中的作用需要进一步研究.
研究的目的:
- 为了研究在Physcomitrium patens中受伤诱导的细胞重编程期间的actin丝和微管的时空动力学.
- 阐明机械感知,细胞骨动力学和细胞重编程在植物再生中的联系.
- 了解细胞骨网络在引导细胞在再生过程中的行为中的作用.
主要方法:
- 激光诱导的伤害 Physcomitrium patens. 这是一种激光.
- 实时成像用于观察actin丝和微管的动态.
- 细胞骨架网络 (F-actin和微管) 的扰乱,以评估它们在再生中的作用.
主要成果:
- 在受伤部位附近的血膜上观察到F-actin的快速局部积累.
- 微管的动力学显示,受伤后没有立即变化.
- 破坏F-actin显著损害了再生和降低了重新编程速率.
- 微管乱主要影响再生细胞分裂.
- 细胞骨网络脱聚变改变了再生模式,影响了细胞外生长与分裂比和外生长极性.
结论:
- 细胞骨在调节植物再生过程中细胞重编程方面发挥着至关重要的功能作用.
- 受伤后的早期细胞骨两极化指导细胞外生长的两极性.
- 这项研究为植物从机械损伤中再生的机制提供了新的见解.
相关概念视频
Actin Polymerization and Cell Motility
5.5K
Actin is a family of globular proteins that are highly abundant in eukaryotic cells. It makes up approximately 1-5% of total cell protein concentration. Actin monomers polymerize to form a complex network of polarized filaments, the actin cytoskeleton, that plays a crucial role in many cellular processes, including cell motility, division, endocytosis, and metastasis of cancer cells.
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
5.5K
Cytoskeletal Coordination in Cell Migration
4.9K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
4.9K
Mechanism of Lamellipodia Formation
2.8K
Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
2.8K
Cell Polarization by Rho Proteins
2.8K
Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
2.8K
Cell Migration
5.2K
Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
5.2K
Cell Motility through Blebbing
2.0K
Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
Blebbing Through the Matrix
In multicellular...
2.0K


