相关实验视频

Updated: Sep 18, 2025

Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets

Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets

Published on: July 7, 2010

ΔNp63β 的差异转录活性是由异型特异性的 C-终端编码的

Abby A McCann¹, Morgan A Sammons¹

¹Department of Biological Sciences, The RNA Institute University at Albany, State University of New York, Albany, New York, USA.

Molecular and cellular biology

|June 23, 2025

概括

转录因子p63有多种异型,具有不同的功能. 研究人员发现,ΔNp63β异型调节独特的基因和增强剂活性,突出显示了p63中的C端变异的重要性.

科学领域:

分子生物学分子生物学
遗传学是一个遗传学.
发展生物学发展生物学

背景情况:

p63是一个关键的转录因子,参与发育和疾病.
突变和p63的过度表达与发育缺陷和癌症有关.
了解p63的多样性作用是复杂的,因为它有多种异型.

研究的目的:

调查p63异型 ΔNp63ɑ 和 ΔNp63β 的独特分子和生化活动.
确定C端拼接变体对p63功能的贡献.
阐明p63异构体在生物过程中的不同作用.

主要方法:

在异形特异细胞系上利用RNA测序 (RNA-seq) 和染色体免疫沉测序 (ChIP-seq).
生成并分析了一系列C端突变的ΔNp63β.
基因组结合位点和增强剂相关的组素修饰 (H3K27ac) 在异构体之间进行比较.

主要成果:

与ΔNp63ɑ相比,ΔNp63β调节了共享和独特的基因组.
大多数ΔNp63ɑ和ΔNp63β的基因组结合部位重叠.
与 ΔNp63ɑ 相比,由 ΔNp63β 结合的增强器区域显示出 H3K27ac 的丰富度明显更高.
异形特异性C端域对于调节独特的ΔNp63β活动至关重要.

结论:

p63 C端异型表现出差异性活动,影响基因调节和生物功能.
ΔNp63β 的独特功能是由其独特的C端域介导的.
这些发现为转录因子异形调节的复杂性及其在发育和疾病中的作用提供了新的见解.

关键词:

转录因子是一种转录因子.基因调节基因调节基因调节转录转录是一种转录.

更多相关视频

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

相关概念视频

Master Transcription Regulators

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...

RNA Polymerase II Accessory Proteins

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...

General Transcription Factors

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...

Chromatin Structure Regulates pre-mRNA Processing

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...

Exon Recombination

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon...

The Eukaryotic Promoter Region

The Eukaryotic Promoter Region

The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences. The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...