什么是eDNA方法标准化,为什么我们需要它?
Susanna Theroux1, Adam Sepulveda2, Cathryn L Abbott3
1Southern California Coastal Water Research Project, Costa Mesa, CA, USA.
概括
环境DNA (eDNA) 方法正在快速发展,但缺乏标准化阻碍了数据共享. 本评论澄清了术语,并促进了eDNA方法开发和采用的路线图,以满足环境监测需求.
科学领域:
- 环境科学环境科学
- 分子生物学分子生物学
- 生态生态学 生态生态学
背景情况:
- 在过去的二十年中,环境DNA (eDNA) 科学有了显著的增长,导致了多样化的采样和分析方法.
- 方法开发往往在机构内部孤立,限制了数据交换和协作进展.
- 在eDNA研究和监管中,关于正式标准与最佳实践指南的应用存在着持续的混乱.
研究的目的:
- 为了澄清eDNA中围绕标准化的术语.
- 区分正式标准和最佳实践指南.
- 讨论环境管理场景如何为eDNA方法的选择提供信息并促进标准化.
主要方法:
- 文献综述和对eDNA当前标准化工作的综合.
- 分析正式标准在监管和研究环境中的作用.
- 对正式的eDNA方法标准进行案例研究审查,以告知管理行动.
主要成果:
- 正式标准和最佳实践指南在eDNA中发挥着不同的但互补的作用.
- 环境管理需求可以指导标准化eDNA协议的开发和采用.
- 现有的例子表明,正式的eDNA标准在环境管理中的成功应用.
结论:
- 电子DNA方法的标准化对于推动该领域的发展和实现大规模生物监测至关重要.
- 需要为eDNA方法开发制定协调的路线图,以解决标准化差距.
- 越来越多地采用标准化的eDNA方法,将加速我们应对当前环境挑战的能力.
更多相关视频
04:50Author Spotlight: Biological Standardization to Ensure Reproducibility and Harmonization in Research
Published on: August 4, 2023
1.2K
07:57Quantitative Detection of Trace Explosive Vapors by Programmed Temperature Desorption Gas Chromatography-Electron Capture Detector
Published on: July 25, 2014
20.1K
相关概念视频
Data Validation
263
Method validation is a crucial process in analytical chemistry designed to confirm that a given method consistently produces reliable and high-quality results. This process is essential when a method is applied to different sample matrices or when procedural modifications are made, ensuring that the results meet acceptable standards across various applications.
Key parameters for method validation include:
Key parameters for method validation include:
263
Methods of Documentation V: CBE
988
Charting by Exception, or CBE, is a method of documentation used in healthcare, particularly in nursing, that focuses on documenting only significant or abnormal findings rather than recording every detail. This approach aims to streamline the documentation process, improve efficiency, and ensure that healthcare providers can quickly identify deviations from normalcy in patient assessments.
In CBE, healthcare professionals establish predefined standards of practice that define what constitutes...
In CBE, healthcare professionals establish predefined standards of practice that define what constitutes...
988
Units and Standards of Measurement
36.4K
A physical quantity is defined either by specifying its measurement method or by stating how it is calculated from other measurements. For example, consider a metallic cube. We might define its mass and dimensions by specifying methods for measuring them, such as using a weighing machine and a meter scale. Then, we could define the volume by stating that it is the cube of its side, and we could calculate the density as the mass divided by the volume.
Measurements of physical quantities are...
Measurements of physical quantities are...
36.4K
Standard Solutions
599
Standard solutions refer to solutions with a precisely known concentration or composition. A primary standard is a highly pure, high molar mass, stable substance that is entirely soluble in water, the most commonly used solvent in analytical chemistry. The primary standard solution can be used to standardize secondary standards, which are substances with known concentrations but are less pure and stable. Standard solutions are essential for achieving accurate and reliable results in analytical...
599
Systematic Error: Methodological and Sampling Errors
2.6K
In the case of systematic errors, the sources can be identified, and the errors can be subsequently minimized by addressing these sources. According to the source, systematic errors can be divided into sampling, instrumental, methodological, and personal errors.
Sampling errors originate from improper sampling methods or the wrong sample population. These errors can be minimized by refining the sampling strategy. Defective instruments or faulty calibrations are the sources of instrumental...
Sampling errors originate from improper sampling methods or the wrong sample population. These errors can be minimized by refining the sampling strategy. Defective instruments or faulty calibrations are the sources of instrumental...
2.6K
Guidelines for Nursing Documentation I
1.2K
Quality documentation and reporting share essential characteristics that ensure they are practical and valuable resources for those who use them. These characteristics are:
Factual:
The following points emphasize the significance of upholding accurate and unbiased documentation in healthcare.
Factual:
The following points emphasize the significance of upholding accurate and unbiased documentation in healthcare.
1.2K
