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基于转录组的EST-SSR标志物的开发用于Radix Ardisia中的遗传分析

Deqiang Ren¹, Wenwen Wu¹, Qinqin Wen¹

¹College of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou, China.

|June 23, 2025

概括

这项研究开发了表达序列标记-简单序列重复 (EST-SSR) 标记,以分析传统族药物Radix Ardisia的遗传多样性. 这些标记有效地识别了Radix Ardisia及其杂物,有助于资源保护和药物安全.

科学领域:

药理学和民族植物学.
分子生物学和遗传学
药用植物研究药用植物研究

背景情况:

拉迪克斯·阿尔迪西亚 (Radix Ardisia) 是一种重要的传统苗族药物,用于喉疾病,来源于各种阿尔迪西亚物种.
随着药用价值的增加和野生资源的减少,需要有效的识别方法来识别Radix Ardisia及其 adulterants.
准确的鉴定对于保护生殖质资源和确保临床药物安全至关重要.

研究的目的:

为Radix Ardisia开发表达序列标记-简单序列重复 (EST-SSR) 的分子标记物.
分析Radix Ardisia种群的遗传结构和多样性.
评估EST-SSR标记的有用性,以区分Radix Ardisia和容易混的物种.

主要方法:

对Ardisia crenata的转录组序列测定,以确定表达序列标记-简单序列重复 (EST-SSR) 位点.
开发和验证EST-SSR原料对的基础.
使用多态EST-SSR标记物的46个Radix Ardisia样本的遗传分析.

主要成果:

总共有32827个SSR位点被确定,并开发了32827对EST-SSR分子标记物.
单核酸和二核酸重复是Ardisia crenata中主要的SSR类型.
51个初始对成功放大了200个多态波段,揭示了Radix Ardisia的高度遗传多样性,可以分为两个种群.
EST-SSR标记有效地区分了Radix Ardisia与容易混合的物种,包括Bailiangjin,Zhushagen和Hongliangsan.

结论:

表达序列标记-简单序列重复 (EST-SSR) 标记器是Radix Ardisia分子识别的有效工具.
开发的标记物可以区分Radix Ardisia与其杂物,支持质量控制和资源管理.
这项研究为基因分析和保护Radix Ardisia提供了宝贵的参考资料.

关键词:

东欧 - 苏联社会主义共和国.基因分析基因分析这种植物名为Radix Ardisia.参考文献参考文献

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