在芯片上引起肠道透性的流动诱导机械调节
Raffaele Mennella1,2, Sara Sibilio1,2, Francesco Urciuolo1,2,3
1Centre for Advanced Biomaterials for Health Care (IIT@CRIB), Istituto Italiano di Tecnologia, Naples, 80125, Italy.
Materials today. Bio
|June 23, 2025
概括
液体剪切应力显著影响肠道屏障功能. 较低的剪切应力增加了细胞透性,并减少了细胞间吸收,突出其在器官芯片模型中的作用.
科学领域:
- 生物医学工程 生物医学工程
- 生理学 生理学 生理学
- 药物开发 药物开发
背景情况:
- 在营养素和药物研究中,肠道体运输至关重要.
- 微流体器官在芯片平台提供优越的生理复制与传统的体外模型相比.
- 流体机械刺激对肠道屏障透性的影响仍然在很大程度上未被探索.
研究的目的:
- 研究不同流体剪切应力对肠道屏障透性和功能的影响.
- 探索分子运输,上皮质结构和屏障完整性的流动诱导的变化.
- 为设计更具生物相关性的器官芯片模型提供信息.
主要方法:
- 开发一个肠上芯片平台.
- 用三种不同的流体剪切应力对肠道细胞进行机械刺激.
- 对细胞外和细胞间传输的评估.
- 阻抗光谱法用于测量上皮质屏障特性.
主要成果:
- 较低的剪切应力导致大约1.5倍更高和更快的细胞透性.
- 较高的剪切应力导致大约3倍低和延迟的跨细胞吸收.
- 阻抗光谱检测显示了变化的紧密结点和双层电阻,以及随着变化的机械应力而增加的电容.
结论:
- 液体剪切应力作为肠道上皮质运输的显著机械调节器.
- 机械力量影响着穿越肠壁的细胞和细胞通路.
- 这些发现适用于生理条件下的肠道运输和其他上皮细胞系统.
相关概念视频
Biasing of Metal-Semiconductor Junctions
907
Biasing metal-semiconductor junctions involves applying a voltage across the junction. Specifically, the metal is connected to a voltage source, while the semiconductor is grounded. This technique is essential for controlling the direction and magnitude of current flow in electronic devices, including diodes, transistors, and photovoltaic cells.
In Schottky junctions, where the semiconductor is n-type, applying a positive voltage to the metal relative to the semiconductor reduces its Fermi...
In Schottky junctions, where the semiconductor is n-type, applying a positive voltage to the metal relative to the semiconductor reduces its Fermi...
907
Enteric Nervous System: Regulation of GI Motor Activity
2.1K
The Enteric Nervous System (ENS) plays a pivotal role in regulating gastrointestinal or GI motor activity. This complex network of nerves, deeply embedded within the gut wall, responds to changes in the gut environment and receives input from both the autonomic nervous system and the central nervous system. By doing so, the ENS operates various programs tailored to the body's nutritional status and needs.
During periods of fasting, the ENS initiates the migrating myoelectric complex, a...
During periods of fasting, the ENS initiates the migrating myoelectric complex, a...
2.1K
Biasing of FET
1.0K
Biasing a Junction Field Effect Transistor (JFET) is crucial for setting operational parameters and ensuring efficient functioning in electronic circuits. JFETs are characterized by using a single carrier type in N-channel or P-channel configurations, where the channel is surrounded by PN junctions. These junctions are central to the device's ability to control current flow.
In an N-channel JFET, the structure consists of N-type material forming the channel on a P-type substrate, with the...
In an N-channel JFET, the structure consists of N-type material forming the channel on a P-type substrate, with the...
1.0K
Gastric Motility
4.5K
Gastric motility is the coordinated contraction and relaxation of stomach muscles that convert ingested food into chyme, a semi-liquid substance ready for further digestion in the intestines. The process begins with the vagus nerve inducing the relaxation of the smooth muscles in the fundus and body of the stomach, allowing these regions to expand and accommodate up to approximately 1.5 liters of food and liquid.
Peristaltic Waves and Chyme Formation
Upon food entry, the stomach initiates...
Peristaltic Waves and Chyme Formation
Upon food entry, the stomach initiates...
4.5K
Mechanical and Chemical Digestion in the Small Intestine
4.9K
The small intestine plays a crucial role in our digestive system, performing both mechanical and chemical digestion.
Mechanical digestion in the small intestine involves movements such as segmentations and migrating motility complexes (MMCs), primarily controlled by the myenteric plexus. Segmentations are localized contractions occurring in areas of the intestine distended by chyme—a mixture of partially digested food. These contractions mix chyme with digestive juices, facilitating...
Mechanical digestion in the small intestine involves movements such as segmentations and migrating motility complexes (MMCs), primarily controlled by the myenteric plexus. Segmentations are localized contractions occurring in areas of the intestine distended by chyme—a mixture of partially digested food. These contractions mix chyme with digestive juices, facilitating...
4.9K
Bioreactor Controls-I
94
Maintaining optimal conditions within fermenters is essential for maximizing microbial productivity and ensuring process efficiency. This lesson focuses on key parameters—temperature, foam, pH, carbon dioxide, oxygen, and pressure—and their precise measurement and control strategies in fermentation systems.Temperature ControlTemperature regulation is critical due to the exothermic nature of many fermentation processes. In small laboratory fermenters, temperature is commonly...
94


