通过使用D-最佳设计的Streptomyces pristinaespiralis NRRL ISP-5338对普里斯胺的固态发酵
Muath Suliman1, Amr S Bishr2, Sally T K Tohamy3
1Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, P.O. Box 61413, Abha, 9088, Saudi Arabia.
Bioprocess and biosystems engineering
|June 23, 2025
概括
这项研究优化了使用固态发酵 (SSF) 的普里斯胺素 (PST) 生产. 优化的SSF条件显著增加了PST产量,为这种宽谱抗生素提供了沉浸液体发酵的有效替代方案.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 发酵技术的发酵技术
背景情况:
- 普里斯蒂纳素 (PST) 是一种具有显著杀菌活性的宽谱链杆菌素抗生素.
- 通过使用传统的浸泡液体发酵 (SLF) 进行*Streptomyces pristinaespiralis*的PST生产具有挑战性,产量相对较低.
- 固态发酵 (SSF) 尚未在PST生产中得到探索.
研究的目的:
- 使用固态发酵 (SSF) 来优化PST生产.
- 通过优化营养和环境因素来评估和提高PST产量.
- 为了比较优化SSF与传统SLF的效率.
主要方法:
- 固体基板的一次性优化 (OFAT) 一个因素,湿成分和化时间.
- 响应表面方法 (RSM) 用于初始pH,化温度和注射剂大小的多因素优化.
- 用初始干基质 (IDS) 的 mg/g 和 mg/mL 量化 PST 生产量.
主要成果:
- 在OFAT优化中,小麦,IPS5和9天的潜伏期被确定为最佳,PST产量从0.395增加到0.467 mg/g IDS.
- 与未优化的SSF相比,RSM统计优化了参数,导致PST产量显著增加2.3倍 (0.910 mg/g IDS).
- 优化的SSF实现了比SLF (0.170 mg/mL) 高出5.35倍的产量 (0.910 mg/g IDS).
结论:
- 固态发酵 (SSF) 是一种高效和简单的方法,可以优化普里斯胺素 (PST) 的生产.
- 优化的SSF条件显著提高PST产量,而不是沉浸液体发酵 (SLF).
- 开发的SSF协议适合扩大PST生产规模.
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