牛卵管环境和组成受到身体能量储备增加的负面影响
Natália Marins Bastos1, Rodrigo Silva Goulart2, Alessandra Bridi1
1Department of Veterinary Medicine, College of Animal Science and Food Engineering, University of São Paulo, São Paulo, Brazil.
PloS one
|June 23, 2025
概括
牛的高体能储备 (BER) 可能导致卵管内胰岛素抵抗,影响早期胚胎发育,并可能降低怀孕率. 这项研究分析了中度与高BER的奶牛的卵管环境变化.
科学领域:
- 生殖生物学 生殖生物学
- 动物科学 动物科学
- 代谢研究研究 代谢研究
背景情况:
- 身体的能量储备 (BER) 影响女性生殖功能.
- 卵巢环境在早期胚胎发育中起着至关重要的作用.
- 了解营养状况对卵巢功能的影响对于改善生育能力至关重要.
研究的目的:
- 研究高体能储备 (BER) 对内罗尔牛卵管环境及其分子组成的影响.
- 为了确定升高的BER是否会导致卵巢外细胞囊泡 (EV) 和基因表达的变化.
- 评估对早期胚胎发育和生育能力的潜在影响.
主要方法:
- 尼罗尔牛被分配到中等 (MBER) 或高 (HBER) 的身体能量储备营养计划中.
- 从具有8细胞胚胎的母牛中收集了卵巢组织和光液,在授精后120小时.
- 分析包括细胞外囊泡分析 (大小,度,miRNA),基因表达 (DEG) 和组织形态学.
主要成果:
- HBER组显示出更高的囊外细胞囊 (AMP-EV) 度和更大的静脉外细胞囊 (IST-EV) 大小.
- AMP-EVs的miRNA配置文件表明与细胞生长,迁移,分化和新陈代谢相关的途径发生了变化,HBER奶牛更容易受到胰岛素调节的影响.
- 无论是EV和细胞分析都显示了与胰岛素代谢相关的途径,这表明HBER奶牛的卵管内胰岛素抵抗. MBER奶牛表现出更大的脉血管化.
结论:
- 身体能量储备 (BER) 增加可能会诱导母牛卵管内胰岛素抵抗.
- 这种情况可能会对卵巢功能和早期胚胎代谢产生负面影响.
- 与高BER相关的卵管胰岛素耐药性可能是导致牛妊娠率下降的因素.
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