固体和浸泡状态发酵的比较外蛋白质学,使用降解纤维素酶降解剂Ascomycete Parascedosporium putredinis NO1 NO1进行发酵
Daniel R Leadbeater1, Alexander T Setchfield1, Adam A Dowle2
1Centre for Novel Agricultural Products, Department of Biology, University of York, Heslington, York YO10 5DD, United Kingdom.
Bioresource technology
|June 23, 2025
概括
固态发酵 (SSF) 与浸泡的真菌发酵 (SmF) 相比,显著提高了真菌酶的产生. 这项研究表明,SSF提高了蛋白质产量和细胞酶活性,用于基纤维素生物质的价值化.
科学领域:
- 生物技术是生物技术.
- 酶学 是一种酶学.
- 菌类学 菌类学是指菌类学.
背景情况:
- 沉浸真菌发酵 (SmF) 是因控制酶生产的标准.
- 菌类自然喜欢高湿度,固体环境,与固态发酵 (SSF) 保持一致.
研究的目的:
- 在SSF和SmF条件下比较Parascedosporium putredinis NO1的分泌体.
- 调查SSF与SmF对酶生产和蛋白质表达的影响.
主要方法:
- 对P. putredinis NO1.1的时间序列外蛋白组学分析.
- 在小麦上使用SSF (35% w/v) 和SmF (1.5% w/v) 种植.
主要成果:
- SSF产生的蛋白质产量是SmF的六倍,具有可比的峰酶活性.
- 在SSF分泌体中,CAZYme家族多样性较低,但细胞酶家族 (GH1,GH3,GH6,GH7) 的上调调高.
- SmF表现出挥发性蛋白质表达,而SSF表现出时间稳定性.
结论:
- 与SmF相比,SSF显著改变了P. putredinis NO1秘密组合和活性.
- SSF是一种可行的策略,可以增加真菌酶产量,用于基纤维素生物质价值化.
关键词:
生物经济是生物经济.生物技术是生物技术.卡西姆是我的一个.酶生产 酶生产菌的真菌是一种.红细胞纤维素酸 (Lignocellulose) 是一种纤维素.固态发酵固态发酵是什么潜水发酵的发酵方式更多相关视频
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