治疗点疟疾诊断的技术:一个范围审查
Florinda Coro1, Carmelo De Maria2,3, Valentina D Mangano4
1Department of Information Engineering and Research Centre 'E. Piaggio', University of Pisa, Pisa, Italy.
Infectious diseases of poverty
|June 23, 2025
概括
本次审查强调了在资源较低的环境中需要更好的疟疾点诊断. 虽然存在许多技术,但很少有符合有效现场使用的ASSURED标准,这表明显微镜的进步是关键.
科学领域:
- 医学诊断 医学诊断 医学诊断
- 寄生虫学的寄生虫学
- 公共卫生 公共卫生
背景情况:
- 疟疾仍然是一个重要的全球卫生问题,特别是在缺乏可访问诊断的低资源环境 (LRS) 中.
- 在这些地区,临床诊断 (IVD) 对于早期发现和治疗疟疾至关重要.
- 本范围审查评估了目前的疟疾IVD技术与ASSURED标准对比,以确定差距并加速LRS的开发.
研究的目的:
- 综合审查现有的疟疾IVD技术.
- 根据ASSURED标准,评估它们在LRS中适用于POC的适用性.
- 为了指导未来开发有效的疟疾诊断的流行地区.
主要方法:
- 在PubMed和Web of Science (2003-2023) 中对疟疾POC诊断设备进行系统的文献搜索.
- 对生物标志物,标本,分析方法和读取技术的识别记录的分析.
- 基于七个ASSURED标准和技术准备水平的技术排名.
主要成果:
- 分析了118个记录,免疫测试 (41.5%) 和循环介导同热放大 (LAMP,22.8%) 是最常见的.
- 循环介导的同热放大 (LAMP) 和聚合酶链反应 (PCR) 显示出最高的灵敏度 (检测极限).
- 尽管专注于POC,但很少有研究 (8%) 明确地解决了ASSURED标准,这表明整体兼容性很低.
结论:
- 满足疟疾POC诊断的ASSURED标准仍然是一个挑战.
- 尽管有局限性,光学显微镜是黄金标准,因为它的准确性和最近在低成本设备和机器学习集成方面的进步.
- 进一步投资改进显微镜技术为改善LRS的POC疟疾诊断提供了强大的潜力.
相关概念视频
01:25Rapid Identification of Pathogens
MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...
01:29Malaria
Malaria pathogenesis in humans reflects a delicate interplay between parasite biology and host response. Clinical illness reflects a host’s immune response to the parasite’s asexual replication cycle, which is often asymptomatic in individuals with partial immunity. From the parasite's perspective, transmission between mosquito and human with minimal host pathology is evolutionarily advantageous. Among the six Plasmodium species infecting humans, P. falciparum and P. vivax dominate in global...