Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Labeling DNA Probes03:31

Labeling DNA Probes

8.4K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.4K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Anionic Polymerization of O‑Benzyl and O-<i>tert-</i>Butyldimethylsilyl Dienes Derived from Myrcene to Yield Functional Polyenes.

ACS omega·2026
Same author

In Situ Lipid Interactions of an Anticancer Metal Complex.

Inorganic chemistry·2026
Same author

Correction: Actin arginylation alters myosin engagement and F-actin patterning despite structural conservation.

The Journal of cell biology·2026
Same author

Multichiral Half-Sandwich Ru(II) and Os(II) Anticancer Complexes Containing a Glutathione Synthesis Inhibitor.

Organometallics·2025
Same author

Actin arginylation alters myosin engagement and F-actin patterning despite structural conservation.

The Journal of cell biology·2025
Same author

StayRose: A photostable StayGold derivative redshifted by genetic code expansion.

The Journal of biological chemistry·2025

相关实验视频

Updated: Sep 18, 2025

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging
10:55

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging

Published on: January 5, 2015

16.4K

单基可点击光体用于体外和体外生物成像.

Raja Mohanrao1,2, Clyde S Pinto1, Andrejus Suchenko1

  • 1Centre for Mechanochemical Cell Biology and Warwick Medical School, Division of Biomedical Science, University of Warwick, CoventryCV4 7AL, UK.

ChemistrySelect
|June 24, 2025
PubMed
概括

新的单基光体为生物成像提供了增强的光物理性能. 这些可点击的分子,来源于四甲甲甲,在体外和体内应用中都表现有前途.

关键词:
生物结合物 生物结合物生物成像是一种生物成像.染色体是一种染色体.光探测器的光探测器单基化物是单基化物.

更多相关视频

Dual Bioluminescence Imaging of Tumor Progression and Angiogenesis
10:56

Dual Bioluminescence Imaging of Tumor Progression and Angiogenesis

Published on: August 1, 2019

8.3K
A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence FUEL
07:04

A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence FUEL

Published on: May 23, 2014

11.7K

相关实验视频

Last Updated: Sep 18, 2025

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging
10:55

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging

Published on: January 5, 2015

16.4K
Dual Bioluminescence Imaging of Tumor Progression and Angiogenesis
10:56

Dual Bioluminescence Imaging of Tumor Progression and Angiogenesis

Published on: August 1, 2019

8.3K
A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence FUEL
07:04

A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence FUEL

Published on: May 23, 2014

11.7K

科学领域:

  • 有机化学 有机化学
  • 光物理学的光学物理学
  • 生物医学成像学 生物医学成像学

背景情况:

  • 新型光体的开发对于推进生物成像技术至关重要.
  • 微型化,单基光体在合成和应用方面具有潜在的优势.
  • 四甲甲乙烯 (tetrafluoroterephthalonitrile) 作为合成功能性有机分子的多功能前体.

研究的目的:

  • 报告一系列小型化的,可点击的单基光体.
  • 研究这些光体的结构-性质关系,重点关注光物理特征.
  • 为了证明这些新型光体在生物成像应用中的实用性.

主要方法:

  • 合成来自四二二二二二的光体.
  • 光物理性质的表征,包括吸收和发射光谱.
  • 合成的光体的蛋白质结合.
  • 在体外和体内生物成像模型中评估光素的性能.

主要成果:

  • 一系列小型化的,可点击的单基光体成功合成.
  • 与具有二[1,4]氨酸骨架的光体相比,具有四基诺素骨架的光体显示出更好的光物理特性.
  • 在四基诺素衍生物中,增强的电子外定位有助于它们的卓越性能.
  • 合成的可点击光体在蛋白质结合后的体外和体内生物成像中都得到了有效的应用.

结论:

  • 微型化,单基的光体来源于四二二二二,代表了一类有前途的成像剂.
  • 四基诺素支架提供了一种可行的策略,用于增强光的光物理性质.
  • 这些易于合成和可点击的光体适合基于蛋白质的生物成像,促进各种生物研究.