迪克耶亚·阿纳纳蒂斯 (Dickeya ananatis) sp. sp. 是一种的植物. nov.,从 (Ananas comosus) 中分离出来的花粉分解性细菌
Shefali Dobhal1, Nicole Hugouvieux-Cotte-Pattat2, Dario Arizala1
1Department of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Honolulu, HI, USA.
概括
一种新的细菌物种Dickeya ananatis被提议用于导致心脏腐烂的菌株. 基因组和遗传学分析证实它与Dickeya zeae复合体的区别,揭示了一个独特的固基因集群.
科学领域:
- 细菌学和植物病理学
- 基因组学和植物遗传学
- 微生物分类学 微生物分类学
背景情况:
- 细菌物种Dickeya zeae表现出复杂的物种内集群,其中一些菌株可能代表新物种.
- 由Dickeya物种引起的细菌性心脏腐烂会影响 (Ananas comosus) 产量,需要准确地对致病原体进行分类鉴定.
研究的目的:
- 在夏威夷和马来西亚从显示细菌心脏腐烂的松植物中分离的Dickeya菌株进行基因组学特征和分类.
- 为了确定这些菌株是否代表Dickeya属内的独特物种.
主要方法:
- 从中分离出来的七个Dickeya菌株的全基因组测序.
- 使用平均核酸标识 (ANI) 和数字DNA-DNA杂交 (dDDH) 的比较基因组分析.
- 植物遗传学分析包括16S rRNA,gapA,十个家政基因的多位置序列分析 (MLSA),以及全基因组/泛基因组比较.
- 特定基因集群的识别和表征,包括固定基因.
主要成果:
- 基因组数据 (ANI和dDDH) 表明,七种衍生菌株属于单一物种,但与已知的Dickeya zeae,Dickeya oryzae和Dickeya parazeae类型菌株不同.
- 遗传学分析始终将这些菌株置于一个单独的单类,Dickeya oryzae是最接近的亲属.
- 一个新的28基因固基因集群,类似于Klebsiella spp.中发现的,在所有七个菌株中都被确定.
结论:
- 多相证据支持对一种新的细菌物种Dickeya ananatis sp.的划分. 对于与相关的菌株.
- 拟议的物种与Dickeya zeae复合体的其他成员有所区别.
- 固定基因集群的存在可能表明与宿主植物相互作用相关的独特代谢能力.
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