Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Labeling DNA Probes03:31

Labeling DNA Probes

8.4K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.4K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Dual tumour-myeloid targeting of glioblastoma with GPNMB CAR-T cells.

Nature·2026
Same author

Selectively targeting inosine monophosphate dehydrogenase-2 impairs brain metastatic potential while preserving immune cell function.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

TP63 expression correlates with mesenchymal-like gene signature in patient-derived recurrent glioblastoma cells.

Biochemical and biophysical research communications·2026
Same author

uPAR is highly expressed in recurrent glioblastoma and represents a candidate CAR T cell target.

Science translational medicine·2026
Same author

Generation of Allogeneic CAR-T Circumvents Functional Deficits in Patient-Derived Autologous Product for Glioblastoma.

International journal of cancer·2026
Same author

Targeting SRC enhances differentiation and promotes multifaceted cell death mechanisms in recurrent group 3 medulloblastoma.

Cell death & disease·2026
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
查看所有相关文章

相关实验视频

Updated: Sep 18, 2025

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay
13:10

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Published on: June 9, 2017

10.1K

近距离连接试验.

Shan Grewal1,2, Shawn C Chafe2,3, Chitra Venugopal2,3

  • 1Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON, Canada.

Methods in molecular biology (Clifton, N.J.)
|June 24, 2025
PubMed
概括
此摘要是机器生成的。

近距离结合试验 (PLA) 可视化了瘤细胞中的蛋白质相互作用. 这种方法可以在现场检测蛋白质的同位化,从而提供对脑瘤进展的见解.

关键词:
在本地化,在本地化.光显微镜的光学显微镜.在现场测试.蛋白质蛋白质相互作用空间组织空间组织.

更多相关视频

Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay
09:18

Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay

Published on: October 20, 2018

7.6K
Author Spotlight: Combining Proximity Ligand Assay with Gamma-H2AX Staining to Characterize Protein Interactions in DNA Damage Response
09:39

Author Spotlight: Combining Proximity Ligand Assay with Gamma-H2AX Staining to Characterize Protein Interactions in DNA Damage Response

Published on: August 2, 2024

603

相关实验视频

Last Updated: Sep 18, 2025

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay
13:10

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Published on: June 9, 2017

10.1K
Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay
09:18

Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay

Published on: October 20, 2018

7.6K
Author Spotlight: Combining Proximity Ligand Assay with Gamma-H2AX Staining to Characterize Protein Interactions in DNA Damage Response
09:39

Author Spotlight: Combining Proximity Ligand Assay with Gamma-H2AX Staining to Characterize Protein Interactions in DNA Damage Response

Published on: August 2, 2024

603

科学领域:

  • 生物化学 生物化学
  • 分子生物学分子生物学
  • 细胞生物学 细胞生物学

背景情况:

  • 蛋白质与蛋白质之间的相互作用对于细胞功能至关重要.
  • 了解这些相互作用是解读疾病机制的关键.
  • 近距离结合试验 (PLA) 为检测这些相互作用提供了一种敏感的方法.

研究的目的:

  • 详细说明在患者衍生的瘤细胞中执行PLA的协议.
  • 用光显微镜在现场可视化蛋白质的同位化.
  • 为了强调PLA在研究脑瘤进展中的实用性.

主要方法:

  • 使用PLA与DNA探针连接到针对蛋白的抗体.
  • 在40nm附近检测蛋白质复合体.
  • 采用滚动圆放大用于信号生成和光显微镜用于检测.

主要成果:

  • 在患者衍生瘤细胞中成功可视化蛋白质的同位化.
  • 在复杂的生物样本中证明PLA的高特异性和灵敏性.
  • 精确捕捉蛋白质与蛋白质相互作用和细胞内的空间组织.

结论:

  • 在现场研究蛋白质相互作用时,PLA是一种有价值的工具.
  • 描述的协议促进了PLA在瘤细胞研究中的应用.
  • PLA为脑瘤进展的分子机制提供了关键的见解.