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转录因子GATA5通过在氨压力下调节Penaeus vannamei中的miR-2765来增强自

Feifei Wang¹, Binbin Lv², Lin Huang³

¹Key Laboratory of Fishery Drug Development of Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Aquatic Animal Immunology and Sustainable Aquaculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China; Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou 510631, China.

International journal of biological macromolecules

|June 24, 2025

概括

我们在中发现了PvGATA5,它可以在压力下增加免疫细胞数量和自. 这种转录因子通过调节miR-2765来改善的生存,为无脊椎动物的免疫提供了洞察力.

科学领域:

分子生物学分子生物学
免疫学免疫学免疫学
甲动物研究研究

背景情况:

GATA转录因子对于细胞发育和应激反应至关重要.
GATA在甲动物免疫调节中的作用尚不清楚.
氨压力对鱼等水生动物产生重大影响.

研究的目的:

为了识别和描述Penaeus vannamei () 中一种类似GATA5的蛋白质.
研究PvGATA5在免疫反应和应激耐受性中的功能.
阐明PvGATA5在调节基因表达中的分子机制.

主要方法:

对PvGATA5.5的识别和序列分析.
遗传学分析以确定进化关系.
在血细胞中的表达分析.
功能性测定包括血细胞计数,自评估,双化酶记者测定,ChIP-PCR和EMSA.

主要成果:

在Penaeus vannamei中发现了一种类似GATA5的蛋白质PvGATA5,具有保存的DNA结合域.
在血细胞中PvGATA5表达最高,在氨压力下增强血细胞计数和自.
PvGATA5直接与miR-2765的促进体结合,从而对其表达进行负面调节.

结论:

在中,PvGATA5在促进自和维持造血平衡方面发挥着至关重要的作用.
PvGATA5-miR-2765通路对于减轻氨引发的压力损伤至关重要.
这项研究提供了关于无脊椎动物免疫和应激反应中的GATA功能的基础知识.

关键词:

氨的气是氨中的一种.自自是一种自的过程.GATA5 GATA5是什么意思类是什么意思?类是什么意思?

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