患有急性尾炎的儿童的腹腔液中独特的微生物特征和与严重程度相关的变化
Tsubasa Aiyoshi1, Tomo Kakihara2, Eiichiro Watanabe3
1Department of Pediatric Surgery, Institute of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan; Laboratory for Symbiotic Microbiome Sciences, RIKEN Center for Integrative Medical Sciences, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan.
概括
患有尾炎的儿童的腹腔液微生物群与尾膜微生物群不同,并且随着尾炎的严重程度而变化. 这影响了对尾切除术后腹内瘤发展的理解.
科学领域:
- 微生物学 微生物学
- 儿科手术 儿科手术
- 胃肠病学 胃肠病学
背景情况:
- 尾切除术后的腹内 (IAA) 来自残留的细菌.
- 在儿科急性尾炎 (AA) 中,腹膜液 (PF) 微生物群缺乏使用培养独立方法的彻底表征.
- 这项研究研究了使用下一代测序的儿科AA中的PF微生物群.
研究的目的:
- 在患有急性尾炎的儿童中描述腹腔液微生物群.
- 为了比较PF微生物群与尾微生物群.
- 探索PF微生物群组成和尾炎严重程度之间的相关性.
主要方法:
- 对21名小儿AA患者进行尾切除的前性观察性研究.
- 采集腹液 (PF) 和尾光样.
- 用于微生物群分析的16S rRNA amplicon测序.
主要成果:
- 关键的PF细菌属包括Bacteroides,Parvimonas,Prevotella,Streptococcus,Blautia和Fusobacterium (≥5%的相对丰富度).这些细菌属中,最常见的细菌是杆菌,杆菌和杆菌.
- PF微生物组合与血清C反应蛋白 (CRP) 水平显著相关 (P=0.011).
- 在简单尾炎 (SA) 和复杂尾炎 (CA) 之间,以及在PF和尾膜之间,观察到微生物群组成和多样性的显著差异.
结论:
- 腹膜液微生物群与尾膜相比,具有不同的组成.
- PF微生物群的变化与AA严重程度相关,反映了炎症反应 (CRP) 和病理分类 (SA与CA).
- 这些发现为手术后腹部内的病变产生提供了新的见解.
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