生物炭诱导的定数感应通过加强直接的物种间电子转移来增强甲生产
Yu Li1, Chenlong Fan1, Lingsen Liu1
1Key Lab of Environmental Engineering, Shaanxi Province, Xi'an University of Architecture and Technology, No. 13 Yanta Road, Xi'an 710055, PR China.
Bioresource technology
|June 24, 2025
概括
生物炭通过增加N-同类素乳 (AHLs) 来增强直接的物种间电子转移 (DIET). 这通过改善微生物电子交换和丰富Geobacter和Methanosaeta等关键细菌来提高甲生产.
科学领域:
- 环境微生物学环境微生物学
- 生物技术是生物技术.
- 无氧消化消化无氧消化
背景情况:
- 直接的物种间电子转移 (DIET) 对无氧环境中的微生物代谢至关重要.
- 已知生物炭会影响微生物群落,并增强甲基生成等过程.
- 在生物炭介导的饮食中,N-同类素乳 (AHLs) 的作用尚未完全理解.
研究的目的:
- 阐明AHLs在生物炭促进饮食中的特定作用.
- 研究生物炭添加如何影响AHL分泌和随后的微生物相互作用.
- 确定AHLs对甲生产率和微生物群落结构的影响.
主要方法:
- 在无氧消化系统中添加生物炭.
- 测量挥发性脂肪酸降解和甲化速率.
- 使用已建立的分析技术量化AHLs.
- 细胞外聚合物物质 (EPS) 组成和电子转移能力的分析.
- 使用16S rRNA测序进行微生物社区分析.
主要成果:
- 添加生物炭加速了挥发性脂肪酸的降解,使最大甲化速率增加了24.97%.
- 生物炭显著增加了大约2.7倍的AHL分泌.
- 增加的AHL促进了c型细胞染色体和酸类物质的合成,增强了EPS电子转移.
- 生物炭诱导的AHLs丰富了饮食伙伴,增加了Geobacter和Methanosaeta的相对丰度.
结论:
- 生物炭诱导的AHLs通过提高EPS电子传输能力来增强饮食.
- AHLs促进了电活性微生物的丰富,例如Geobacter和Methanosaeta.
- 这些机制共同提高了无氧消化系统中的甲产量.
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