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Bioremediation is the use of prokaryotes, fungi, or plants to remove pollutants from the environment. This process has been used to remove harmful toxins in groundwater as a byproduct of agricultural run-off and also to clean up oil spills.
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Measuring Phosphorus Release in Laboratory Microcosms for Water Quality Assessment
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通过单细胞RACS-Culture用于合理的生态系统工程,采矿可靠的现场积生物.

Xiaoyan Jing1, Yanhai Gong2, Yishang Ren2

  • 1College of Biological Engineering, Qingdao University of Science and Technology, Qingdao, Shandong, PR China; Single-Cell Center, CAS Key Laboratory of Biofuels, Shandong Key Laboratory of Energy Genetics and Shandong Energy Institute, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong, PR China.

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概括

我们开发了一种新的方法来寻找和使用微生物来进行生态系统工程. 该战略成功地发现了一种新型聚酸盐积聚生物 (PAO),该生物显著改善了废水处理中的去除.

关键词:
生态系统工程生态系统工程积有机体 (PAO) 是一种积有机体.单细胞拉曼激活细胞分类和培养 (scRACS-Culture) 进行.污水处理 污水处理 污水处理

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科学领域:

  • 微生物学 微生物学
  • 环境工程 环境工程
  • 生物技术是生物技术.

背景情况:

  • 理性生态系统工程受到在现场识别和培养关键微生物的困难所限制.
  • 有效的微生物采矿需要基于其本土环境中的实际代谢功能来分析和分离生物的方法.

研究的目的:

  • 开发和展示一种以实地代谢驱动的分类,培养和增量 (IMSCA) 策略.
  • 在现场开采聚酸盐积聚生物 (PAO) 以提高废水处理.

主要方法:

  • 利用拉曼激活细胞分类与单细胞培养 (scRACS-Culture) 结合,用于无标签的分析和隔离.
  • 使用拉曼光谱学直接从环境样本中量化评估单细胞聚酸盐积累活动.
  • 在现场发现的高活性PAO进行培养和表征.

主要成果:

  • 鉴定Micrococcus luteum CI5-8是一种新型的PAO,具有独特的生理特征 (例如,没有无氧酸盐释放,依赖糖原).
  • 与纯种植相比,这种新型PAO在现场表现出明显更高的活性.
  • 采用MCI5-8的重新设计的废水处理工艺使无氧-无氧-有氧 (AAO) 反应堆的去除效率从45%提高到89%.

结论:

  • 该IMSCA战略使有效的生物资源采矿通过"屏幕第一,培养第二"的方法,基于现场代谢.
  • 这种方法广泛适用于合理的生态系统工程和发现新的微生物功能.
  • 发现的新型PAO为改善废水处理中的去除提供了有希望的解决方案.