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在植物中,贝他林生物合成途径与细菌二氧化原酶的补充

María Alejandra Guerrero-Rubio¹, Hester Sheehan¹, Samuel F Brockington¹

¹Department of Plant Sciences, University of Cambridge, Cambridge, United Kingdom.

|June 24, 2025

概括

细菌酶可以产生称为贝他林的植物颜料. 一种细菌的4,5-L-DOPA-extradiol-dioxygenase (DODA) 酶在植物中成功合成了贝他林,支持了融合进化理论.

科学领域:

生物化学生物化学
植物科学植物科学
微生物学微生物学

背景情况:

贝塔林是一种植物颜料,通过4,5-L-DOPA-extradiol-dioxygenase (DODA) 合成.
在植物,真菌和细菌中发现DODA酶,具有显著的结构差异.
跨王国的DODA酶的功能交换和补充在很大程度上仍未被探索.

研究的目的:

调查细菌DODA酶是否可以功能地补充植物贝他林生物合成途径.
为了确定是否可以使用非植物DODA酶在植物中产生贝他林.
探索DODA酶活动在不同王国的进化影响.

主要方法:

在Nicotiana benthamiana中,细菌DODA基因的短暂表达.
使用高性能液体染色学-电子喷射电离-质谱法 (HPLC-ESI-MS) 分析贝塔林生产.
细菌DODA活性与植物DODA酶的比较.

主要成果:

在植物中,Gluconacetobacter diazotrophicus DODA酶成功地产生了贝塔胺酸和多种贝塔林.
鉴定出vulgaxanthin I 是浸叶子中占主导地位的贝他林色素.
细菌酶有效地补充了植物贝他林生物合成途径.

结论:

来自植物王国以外的功能性贝他林形成酶可以补充植物通路.
这一发现支持了贝他林形成活动的融合进化假设.
跨王国的酶交换和功能补充对于贝他林生物合成是可能的.

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