Search research articles

关于 JoVE

概览领导团队博客 JoVE 帮助中心

作者

出版流程编辑委员会范围与政策同行评审常见问题投稿

图书馆员

用户评价订阅访问资源图书馆顾问委员会常见问题

研究

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments 存档

教育

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual 教师资源中心教师网站

使用条款与条件

相关概念视频

Transducer Mechanism: Enzyme-Linked Receptors

Transducer Mechanism: Enzyme-Linked Receptors

Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序

Same author

Mining and engineering of ene-reductases from marine sediment metagenome for prochiral ACE inhibitor synthesis.

Applied and environmental microbiology·2026

Same author

On the sulfide oxidation to sulfoxides using sodium orthovanadate in water.

Chemical communications (Cambridge, England)·2025

Same author

Enantioselectivity in Vanadium-Dependent Haloperoxidases of Different Marine Sources for Sulfide Oxidation to Sulfoxides.

Marine drugs·2024

Same author

Marine-Derived Fungi as a Valuable Resource for Amylases Activity Screening.

Journal of fungi (Basel, Switzerland)·2023

Same author

Recent Advances in the Synthesis of Marine-Derived Alkaloids via Enzymatic Reactions.

Marine drugs·2022

Same author

Enzymatic synthesis of enantiopure alcohols: current state and perspectives.

RSC advances·2022

Search research articles

相关实验视频

Updated: Sep 18, 2025

Combining 3D Magnetic Force Actuator and Multi-Functional Fluorescence Imaging to Study Nucleus Mechanobiology

Combining 3D Magnetic Force Actuator and Multi-Functional Fluorescence Imaging to Study Nucleus Mechanobiology

Published on: July 5, 2022

磁性多功能模块多相机动模仿多酶魔术的多相机动

Yongyi Zeng¹, Xinjian Yin¹, Yating Zou¹

¹School of Marine Sciences, Sun Yat-Sen University, Zhuhai 519080, China.

Journal of agricultural and food chemistry

|June 24, 2025

概括

这项研究引入了一种用于联合调动多种蛋白质的新的插电显示方法,从而能够精确控制酶排列和负载比率,从而实现高效的生物催化.

关键词:

捕获者/标记者对的组合在HaloTag中使用HaloTag.细胞溶解酸细胞溶解酸.酶协同调动酶协同调动磁性微粒是指磁性微粒的微粒.

更多相关视频

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System

Published on: June 13, 2010

Simultaneous Electrical and Mechanical Stimulation to Enhance Cells' Cardiomyogenic Potential

Simultaneous Electrical and Mechanical Stimulation to Enhance Cells' Cardiomyogenic Potential

Published on: January 18, 2019

相关实验视频

Last Updated: Sep 18, 2025

Combining 3D Magnetic Force Actuator and Multi-Functional Fluorescence Imaging to Study Nucleus Mechanobiology

Combining 3D Magnetic Force Actuator and Multi-Functional Fluorescence Imaging to Study Nucleus Mechanobiology

Published on: July 5, 2022

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System

Published on: June 13, 2010

Simultaneous Electrical and Mechanical Stimulation to Enhance Cells' Cardiomyogenic Potential

Simultaneous Electrical and Mechanical Stimulation to Enhance Cells' Cardiomyogenic Potential

Published on: January 18, 2019

科学领域:

生物技术是生物技术.
生物催化剂是一种生物催化剂.
蛋白质工程是指蛋白质工程.

背景情况:

在纳米支上联合调动多种蛋白质模仿自然复合体,但在空间控制和蛋白质净化方面面临挑战.
现有的方法通常需要纯化蛋白质,并且缺乏对不同酶的排列和加载比率的精确控制.

研究的目的:

开发一种新的,可调节的协同动员策略,用于精确地控制多种蛋白质的空间布局和负载比控制.
创建一个高效的平台,直接从细胞溶解物进行酶固定,减少处理时间和成本.

主要方法:

开发了一个插件和显示策略,将Catcher/Tag对与HaloTag技术结合起来.
设计了一个磁性多功能模块平台,用于顺序和可调节的酶捕获.
通过HaloTag和补充的Catcher/Tag系统,利用了含有chloroalkane功能的磁珠来固定酶.

主要成果:

通过不同的Catcher/Tag组合和顺序,通过不同的Catcher/Tag组合和顺序,实现了对酶空间布局和加载比的精确控制.
证明了高蛋白载荷能力,并保留了固定酶的催化活性.
直接从细胞溶解物中成功捕获酶,大大简化了固定化过程.

结论:

开发的插电显示系统为联合动员策略提供了重大进步.
这种方法为创建多蛋白系统提供了精确的控制,效率和成本效益.
该技术对生物技术,生物催化剂和生物医学领域的各种应用具有前景.