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胃化过程中的动态WT1表达指定了腹膜平滑肌的命运,独立于中肌的命运
Suad H Alsukari1, Huei Teng Ng1, Lilly Lang1
1Department of Women's and Children's Health, Institute of Life Course and Medical Sciences, Faculty of Health and Life Sciences, University of Liverpool, Liverpool L69 3BX, UK.
概括
在小鼠胃流动过程中,威尔姆斯瘤蛋白 (WT1) 表达意外地指定了光滑肌肉前体细胞. 这项研究揭示了WT1.
科学领域:
- 发育生物学是发展生物学.
- 细胞谱系的追踪 细胞谱系的追踪
- 分子胚胎学分子胚胎学
背景情况:
- 之前的研究将威尔姆斯瘤蛋白 (WT1) 与小鼠肠道中介质和血管光滑肌肉细胞 (vSMC) 血统联系起来.
- 有证据表明,肠道的vSMCs起源于中皮层.
研究的目的:
- 研究WT1在小鼠早期发育过程中的作用,特别是胃流动.
- 确定血管光滑肌细胞 (vSMCs) 和内脏光滑肌细胞 (visSMCs) 的起源.
主要方法:
- 在小鼠胚胎中追踪他莫西芬诱导的遗传血统.
- 从胃化阶段胚胎中分析单细胞RNA测序数据集.
- 同焦显微镜可视化Wt1的表达和同定位与光滑肌肉标记物.
- 在胃化过程中Wt1的有条件淘汰.
主要成果:
- 在老鼠胃化过程中,WT1在侧板介质体中意外地表达,指定SMC前体细胞.
- 血统追踪证实WT1表达细胞会导致胎儿中肠vSMC和visSMC,但不是中细胞.
- 在胃化阶段胚胎中,WT1的表达先于,并且独立于内脏间皮形成.
- 胚胎日 (E) 7.5 的 WT1 淘汰会影响 E12.5.5 的肠道血管化.
结论:
- 在消化过程中,WT1在指定光滑肌肉细胞系的过程中发挥着至关重要的,以前未知的作用.
- 血管和内脏光滑肌细胞起源于WT1表达前体的侧板介质皮,独立于介质皮.
- 这些发现为小鼠胚胎中的光滑肌细胞的发育起源提供了新的见解.
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