同时诊断和各种呼吸道病原体感染的生理分析,使用红外光谱中的多分子特征
Zhouzhuo Tang1, Jing Ni2, Zihao Liu2
1Hangzhou International Innovation Institute, Beihang University, Hangzhou, 311115, China; School of Instrumentation and Optoelectronic Engineering, Beihang University, Beijing, 100191, China.
概括
一个新的诊断平台使用红外光谱学同时检测多种呼吸道感染,如SARS-CoV-2和流感. 这项技术有助于了解病原体与宿主之间的相互作用和疫情防控.
科学领域:
- 生物医学工程 生物医学工程
- 频谱学是一种光谱学.
- 传染性疾病 传染性疾病
背景情况:
- 急性呼吸道感染 (ARI) 是一个重大的全球健康挑战.
- 呼吸道病原体的准确及时诊断对于有效的治疗和控制至关重要.
研究的目的:
- 开发和验证一个通用的诊断平台,用于同时检测和呼吸道病原体感染的生理分析.
- 利用分子特征探索感染引起的生理变化和病理机制.
主要方法:
- 集成便携式减弱总反射-里埃变换红外光谱 (ATR-FTIR) 与先进的化学测量方法.
- 应用红外 (IR) 吸收比率和重要波数范围重叠 (IWRO) 方法用于生物标志物识别和生物分子量化.
- 使用部分最小平方区分分析 (PLS-DA) 验证来自两个临床队列的鼻抽样.
主要成果:
- 该平台成功地区分了由SARS-CoV-2,Mycoplasma pneumoniae,B型流感和健康对照引起的感染.
- 在两个临床队列中实现了95.6%和90.9%的高分类准确率.
- 能够在一次测量中同时检测和对呼吸道病原体进行生理分析.
结论:
- 开发的ATR-FTIR光谱平台为同时检测和分析各种呼吸道病原体提供了一种多功能方法.
- 这项技术为病原体与宿主相互作用提供了关键的见解,增强了未来的流行病准备.
- 扩大了红外光谱在传染病临床诊断中的应用.
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