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Updated: Sep 18, 2025

Simultaneous Quantification of Anti-vector and Anti-transgene-Specific CD8+ T Cells Via MHC I Tetramer Staining After Vaccination with a Viral Vector
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Simultaneous Quantification of Anti-vector and Anti-transgene-Specific CD8+ T Cells Via MHC I Tetramer Staining After Vaccination with a Viral Vector

Published on: November 28, 2018

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人类CMV特异性CD8+ T细胞的表征使用多层单细胞奥米克

Ioanna Gemünd1, Lorenzo Bonaguro2, Matthias Becker3

  • 1Systems Medicine, German Center for Neurodegenerative Diseases (DZNE), Bonn, Germany; Genomics and Immunoregulation, Life and Medical Sciences (LIMES) Institute, University of Bonn, Bonn, Germany; Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Parkville, VIC, Australia; Institute of Virology, Technical University of Munich School of Medicine/Helmholtz Munich, Munich, Germany.

Cell reports methods
|June 24, 2025
PubMed
概括

这项研究提出了一种新的多omics单细胞工作流程,用于分析免疫细胞. 该方法成功地确定了T细胞受体序列,并评估了检测抗原特异性T细胞的试剂.

关键词:
BD 拉普索迪 (Rhapsody) 是一个音乐曲目.CD8 (((+) T细胞是T细胞中的一个.在CMV中使用CMV.CP: 免疫学 免疫学TCR 测序的测序方法抗原特异性T细胞是一种抗原特异性T细胞.在 dCODE 中使用的 dextramers.一个单细胞多细胞的奥米克.系统免疫学 免疫学

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T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
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