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染色体动力学和热应激的转录调节在一个ectothermic鱼
Songqian Huang1,2, Chenyu Yan1,2, Yuan Xu1,2
1Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, People's Republic of China.
Proceedings. Biological sciences
|June 24, 2025
概括
全球变暖导致鱼类的热应激 (HS). 这项研究揭示了CCCTC结合因子 (CTCF) 和激活转录因子4 (ATF4) 如何调节基因表达和染色质可访问性,影响大嘴的内等质网膜应激和亡.
科学领域:
- 环境毒理学环境毒理学
- 分子生物学分子生物学
- 湿热体生理学 湿热体生理学
背景情况:
- 全球变暖和极端高温事件对外热动物构成重大威胁.
- 热应激 (HS) 反应背后的分子机制,特别是关于转录调节和染色质可访问性,在鱼类中尚不完全理解.
研究的目的:
- 为了研究渐变热应激对长嘴 (Micropterus salmoides) 染色质可访问性和转录动态的影响.
- 确定关键的分子参与者和参与热应激反应的调节网络,包括内质网膜 (ER) 应激和亡.
主要方法:
- 在不同热应激条件下分析大鱼大脑,和头组织中的染色质可访问性和转录组.
- 整合cis-regulatory元素分析和转录因子 (TF) 绑定预测,以构建监管网络.
- 使用qPCR和免疫光分析进行实验验证.
主要成果:
- 在头中观察到显著的热应激诱导的亡.
- 确定的关键途径包括内质网膜应激,铁亡和亡.
- 构建了一个涉及259个TF的转录监管网络,突出强调CCCTC-binding factor (CTCF) 和激活转录因子4 (ATF4) 作为中央监管机构.
- 证实CTCF和ATF4能够动态控制导致ER压力和亡的应激反应基因,在HS下增强核CTCF表达.
结论:
- 发现了热应激下鱼类ER压力调节的分子机制.
- 将CTCF和ATF4定位为关键的枢纽调节因子,用于染色体重塑和转录性反应,以应对热应激.
- 提供了关于鱼类面临环境温度上升的适应策略的见解.
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