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使用植物表达系统生产生物活性细胞因子,以从人类多能干细胞中分化心血管细胞

Kozue Murata^1,2, Kanae Takamura¹, Risa Watanabe³

¹Clinical Translational Research Program, Center for Biosystems Dynamics Research, RIKEN, 2-2-3 Minatojimaminami-Cho, Chuo-Ku, Kobe, 650-0047, Japan.

Stem cell research & therapy

|June 24, 2025

概括

植物表达的血管内皮生长因子 (VEGF) 和Activin A为产生心脏细胞提供了一个安全的替代方案. 这些植物衍生的细胞因子有效地诱导来自干细胞的内皮细胞和心肌细胞.

科学领域:

生物技术是生物技术.
干细胞生物学干细胞生物学
再生医学是一种再生医学.

背景情况:

像血管内皮生长因子 (VEGF) 和Activin A这样的生物活性细胞因子对于干细胞分化为血管内皮细胞和心肌细胞至关重要.
使用大肠杆菌或哺乳动物细胞的传统生产方法存在免疫性和病毒污染的风险.

研究的目的:

开发和验证一种植物性表达系统,用于生产VEGF和Activin A.
评估植物表达的VEGF和Activin A在诱导干细胞分化中的生物活性和有效性.

主要方法:

开发一种植物表达系统,用于VEGF和Activin A.
植物表达和商业VEGF与Activin A之间的生物活性比较.
评估植物表达的细胞因子在诱导来自人类多能干细胞的内皮细胞和心肌细胞的疗效.

主要成果:

植物表达的VEGF和Activin A表现出与商业对应物相比具有可比的生物活性.
来自植物的VEGF和Activin A在诱导来自多能干细胞的内皮细胞和心脏细胞方面与人类重组蛋白一样有效.

结论:

植物性表达是生产生物活性细胞因子VEGF和Activin A的可行和安全的替代方案.
植物表达的VEGF和Activin A对基于干细胞的安全和高效的再生医学应用具有显著的前景,特别是在心脏细胞生成方面.

关键词:

在Activin A A活动中.一个心肌细胞.内皮细胞内皮细胞的内皮细胞.诱导多能干细胞 (iPS细胞) (iPSC) 是一种血管内皮生长因子 (VEGF)

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