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Updated: Sep 18, 2025

Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System
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Published on: May 28, 2015

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没有PAM的头目标结合激活了Cas12a的跨裂变活性

Xiaolong Li1, Zixuan Zhu1, Jiani Wu1

  • 1School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.

Nucleic acids research
|June 25, 2025
PubMed
概括

研究人员发现了一种新的方法来激活CRISPR-Cas12a,使用头DNA结构来激活CRISPR-Cas12a,从而绕过了对原空间器相邻动机 (PAM) 的需求. 这一发现使得在对非核酸目标的敏感检测方面有了新的应用.

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