评估口服微生物活性的2,6-二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二
Prem K Sreenivasan1,2, Violet I Haraszthy3
1HITLAB, 3960 Broadway, New York, NY 10032, USA.
Antibiotics (Basel, Switzerland)
|June 25, 2025
概括
这项研究表明,2,6-Dichlorophenolindophenol (DCIP) 可以通过测量颜色变化来快速量化口腔细菌. 这种方法准确地评估了常见的口腔卫生产品的抗菌有效性.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 牙科科学 牙科科学
背景情况:
- 量化口腔细菌对于评估口腔健康至关重要.
- 现有的细菌量化方法可能耗时.
- 开发快速准确的方法对于评估抗菌剂至关重要.
研究的目的:
- 为了评估2,6-二二二 (DCIP) 作为一种色度试剂,用于快速定量口腔细菌.
- 评估使用DCIP减少的口腔卫生产品的抗菌疗效.
- 建立一个具有成本效益的方法来计数口腔微生物.
主要方法:
- 使用2,6-二二二二 (DCIP),一种氧化还原染料,进行色度分析.
- 通过光谱测量测量了DCIP由可活口腔细菌 (例如,Streptococcus mutans,Candida albicans) 的减少.
- 相关的DCIP减少与可活性板数,并评估了微生物无活化的影响.
主要成果:
- 活跃的口腔微生物细胞有效地减少了DCIP,蓝色的损失表明了这一点.
- 在增加可活板数量和DCIP减少之间观察到显著的相关性.
- 与代谢活跃的生物相比,非活化生物体的DCIP减少明显较低.
结论:
- 减少DCIP提供了一种快速,准确和低成本的方法来定量口腔细菌.
- 这种技术有效地评估了口水和牙膏的抗菌特性.
- 该研究提出了一种基于视觉的,可访问的实验室方法,用于口腔生物的计数.
关键词:
在DCIP中,它是DCIP.抗生素 抗生素是一种抗生素.细菌 细菌 细菌是一种细菌.乙烯基化物 cetylpyridinium 化物氧化的含量是多少文化 文化 文化 文化口腔清洗是什么意思氧化氧化还原技术是什么牙膏是一种牙膏.三克洛桑三克洛桑是一种公司的可行性,可行性.更多相关视频
07:23Fluorescence Microscopy Methods for Determining the Viability of Bacteria in Association with Mammalian Cells
Published on: September 5, 2013
44.1K
06:50A Soluble Tetrazolium-Based Reduction Assay to Evaluate the Effect of Antibodies on Candida tropicalis Biofilms
Published on: September 16, 2022
3.2K
相关概念视频
Antimicrobial Effectiveness
175
The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...
175
Redox Titration: Overview
3.5K
Redox titration is a chemical analysis technique used to determine the concentration of an unknown substance by measuring the electron transfer in a redox (reduction-oxidation) reaction. The process involves gradually adding a titrant with a known concentration of an oxidizing or reducing agent, to the analyte, the solution with an unknown concentration, until reaching the endpoint, which indicates the completion of the reaction between the two substances. Ensuring the analyte is in a single...
3.5K
Oxidation of Phenols to Quinones
3.5K
In the presence of oxidizing agents, phenols are oxidized to quinones. Quinones can be easily reduced back to phenols using mild reducing agents. The electron-donating hydroxyl group enhances the reactivity of the aromatic ring, enabling oxidation of the ring even in the absence of an α hydrogen.
o-hydroxy phenols are oxidized to o-quinones and p-hydroxy phenols to p-quinones. Such redox reactions involve the transfer of two electrons and two protons. The reversible redox...
o-hydroxy phenols are oxidized to o-quinones and p-hydroxy phenols to p-quinones. Such redox reactions involve the transfer of two electrons and two protons. The reversible redox...
3.5K
Redox Titration: Other Oxidizing and Reducing Agents
408
Besides iodine, other oxidizing or reducing agents can serve as titrants in redox titrations. Common oxidizing titrants include KMnO4, cerium(IV), and K2Cr2O7. The choice of oxidizing titrants depends on factors like stability, cost, analyte strength, and reaction rate between the analyte and titrant. KMnO4 is a strong oxidizing titrant that reduces from Mn(VII) to Mn(II) in a highly acidic solution, simultaneously oxidizing the analyte to a higher oxidation state. In this case, KMnO4 acts as a...
408
