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从山羊便中分离出来的Enterococcus lactis RB10的基因组特征和安全性评估

Nattarika Chaichana¹, Sirikan Suwannasin¹, Jirasa Boonsan¹

¹Department of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Hat Yai 90110, Songkhla, Thailand.

Antibiotics (Basel, Switzerland)

|June 25, 2025

概括

从山羊便中分离出来的Enterococcus lactis RB10显示出具有抗菌活性和肠道殖民基因的益生菌潜力. 需要进一步的安全评估,但它对食品和健康应用具有低致病风险.

科学领域:

微生物学微生物学
基因组学就是基因组学.
益生菌益生菌益生菌

背景情况:

肠球菌种在胃肠道和各种环境中很常见.
这项研究的重点是从山羊便中分离出来的Enterococcus lactis RB10.

研究的目的:

进行对Enterococcus lactis RB10.10进行全面的基因组和功能分析.
评估该菌株的安全性和益生菌潜力.

主要方法:

使用牛津纳米孔技术 (ONT) 进行全基因组测序.
基因组注释以识别编码序列 (CDS) 和代谢途径.
抗微生物敏感性测试和抗微生物耐药性基因 (ARG) 的鉴定.
分析与粘附,生物膜形成和耐压力相关的基因.

主要成果:

乳腺 E. RB10 的基因组为 2,713,772 bp,含有 38.3% 的 GC 和 3375 个 CDS.
该菌株对关键抗生素敏感,但对氨基糖化物有耐药性,已确定了两个内在的ARG.
RB10 显示抗菌活性对抗 Bacillus cereus 和沙门氏菌 Typhi.
确定了肠道殖民,病原体竞争,细菌素生产和耐压力的基因.

结论:

E. lactis RB10表现出有希望的益生菌特性,包括抗菌活性和肠道殖民的潜力.
该菌株具有较低的致病风险,支持其在食品和健康应用中的潜力.
由于其隔离源 (山羊便) 需要进行额外的安全评估.

关键词:

乳腺肠球菌 (Enterococcus lactis) 是一个细菌.它具有抗菌活性,具有抗菌活性.基因组分析基因组分析潘格诺姆 (pangenome) 是一个名字.安全评估安全评估的安全评估.

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