通过PABPC1调节p53/MDM2通路,LncRNA-ATB有助于严重的孕前
Jiashuo Zhang1, Lan Mei2, Yanping Zhang1
1Department of Obstetrics and Gynecology, Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, NHC Key Laboratory of Chronobiology, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, People's Republic of China.
概括
长非编码RNAATB (lncRNA-ATB) 影响严重孕前症 (sPE) 中的热囊细胞功能. 它调节聚A结合蛋白细胞质蛋白1 (PABPC1),影响p53/MDM2通路,为sPE提供了新的见解.
科学领域:
- 产科和妇科 产科和妇科
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 异常的螺旋动脉重塑引发了孕前 (PE).
- 在严重的PE (sPE) 中观察到降低的胎盘长非编码RNA ATB (lncRNA-ATB) 水平,并抑制了 trofhoblast 细胞功能.
- 对于lncRNA-ATB调节热原体行为的精确机制尚不完全理解.
研究的目的:
- 为了识别与热囊细胞中 lncRNA-ATB 相互作用的蛋白质.
- 阐明在热囊细胞功能中的lncRNA-ATB的生物调节机制.
- 调查lncRNA-ATB在sPE病变发生中的作用.
主要方法:
- RNA-蛋白质拉下测试. 在RNA-蛋白质拉下测试.
- RNA免疫沉降 (RIP) 测试. 检测. 在RNA免疫沉降 (RIP) 测试.
- 试验室研究涉及使用 lncRNA-ATB 和 PABPC1 敲除的热囊细胞系.
主要成果:
- 聚甲基结合蛋白细胞质蛋白1 (PABPC1) 被确定为lncRNA-ATB的直接下游标,其表达受到lncRNA-ATB的积极调节.
- 减少PABPC1表达损害了 trofhoblast 增殖,迁移,入侵和管形成,同时增强了亡.
- 在sPE胎盘中,PABPC1的表达减少,可能通过p53/MDM2通路调节lncRNA-ATB对MDM2的影响,其中PABPC1负面调节p53.
结论:
- PABPC1 作为 lncRNA-ATB 对 trofhoblast 生物功能的影响的关键调解者.
- lncRNA-ATB/PABPC1轴通过p53/MDM2信号通路调节热囊细胞的行为.
- 这些发现提供了对lncRNA-ATB在严重孕前病原发生中的作用背后的分子机制的新见解.
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