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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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基于数值模拟方法的剪切变形细胞计的全面分析.

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科学领域:

  • 生物物理学的生物物理.
  • 细胞力学 细胞力学
  • 流体动力学 流体动力学

背景情况:

  • 细胞变形性对于理解细胞在压力下的行为至关重要.
  • 对影响细胞变形的因素进行系统的研究尚不发达.

研究的目的:

  • 系统地研究各种因素对细胞可变性的影响.
  • 模拟和量化流速,流体粘度,细胞直径和剪切模量对细胞变形的影响.
  • 探索细胞变形性和放松时间之间的关系.

主要方法:

  • 使用一种不可压缩的新胡克式粘弹性固体模型.
  • 纳入了凯尔文-沃伊特模型用于模拟.
  • 在不同的条件下进行细胞变形的系统计算和模拟.

主要成果:

  • 细胞变形与流速和流体粘度正相关,显示了近似的线性关系.
  • 细胞直径对可变性的影响比流速或粘度更大.
  • 增加的模和放松时间导致细胞变形的减少.

结论:

  • 建立了关键物理参数和细胞可变性之间的定量关系.
  • 这些发现为精确控制可变性细胞计中的细胞变形提供了基础.
  • 细胞分析在农业动物健康监测和其他领域的影响.