在患有体征性症状障碍的儿童中,葡萄糖皮质体受体基因的甲基化:一种病例对照研究
Kyoko Hatta1, Masato Kantake1,2, Kyoko Tanaka3
1Department of Pediatrics, Juntendo University Faculty of Medicine, Tokyo 113-0033, Japan.
Epigenomes
|June 25, 2025
概括
葡萄糖皮质体受体 (GR) 基因NR3C1的表观遗传调节在患有体质症状障碍 (SSD) 的儿童中因年龄而异. 甲基化模式与年幼儿童的心理症状相关,但在年长儿童中显示出统一的变化.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 儿童心理学 儿童心理学
- 神经内分泌学神经内分泌学
背景情况:
- 儿童体征性症状障碍 (SSD) 与压力反应和心理社会因素有关.
- 由NR3C1编码的葡萄糖皮质体受体 (GR) 是应激反应的核心.
- 目前尚不清楚NR3C1甲基化与SSD之间的联系.
研究的目的:
- 调查NR3C1外子1F甲基化与儿童体症状障碍 (SSD) 之间的关系.
- 探索与SSD相关的NR3C1甲基化中的年龄相关的表观遗传差异.
- 评估NR3C1甲基化模式与心理评估之间的相关性.
主要方法:
- 在从唾液中获取的无细胞DNA中分析了NR3C1外显子1F甲基化,使用双硫酸片序列.
- 包括34名SSD儿童和29名年龄和性别匹配的对照.
- 使用贝克抑郁症目录II (BDI-II) 和KINDL调查问卷进行心理评估.
主要成果:
- 甲基化模式随年龄而变化. 较小的儿童 (<13) 显示混合甲基化与KINDL和BDI-II分数相关.
- 较年长的儿童 (≥13) 均高NR3C1甲基化,SSD组的甲基化明显高于对照组.
- 患有SSD的老年儿童有更多的正静态失调和更长的疾病持续时间.
结论:
- 在SSD中NR3C1表观遗传调节是年龄依赖的.
- 较小的儿童表现出CpG特定的甲基化相关性与心理症状.
- 较大的孩子表现出均的超甲基化,可能表明累积压力,自主功能障碍和内化障碍.
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