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通过代谢工程来生产乳酸盐的Scheffersomyces stipitis

Angela Matanović¹, Nenad Marđetko², Ana Slišković¹

¹Laboratory for Biology and Microbial Genetics, Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia.

Journal of fungi (Basel, Switzerland)

|June 25, 2025

概括

改造的酵母 Scheffersomyces stipitis 从纤维素生物质中产生乳酸. 改造后的菌株从葡萄糖和果糖中获得了高的乳酸盐产量,显示出对工业应用的前景.

科学领域:

生物技术是生物技术.
工业微生物学工业微生物学
代谢工程是代谢工程.

背景情况:

乳酸盐是一种有价值的工业化学品.
Scheffersomyces stipitis是一种能够发酵六和五酵母的酵母,使其成为细胞生物质转化的一个有希望的宿主.
S. stipitis 不能自然产生乳酸,因此需要基因工程来生产.

研究的目的:

为了工程 Scheffersomyces stipitis生产L-乳酸盐.
评估工程化S. stipitis在从纤维素糖 (葡萄糖和糖) 中生产乳酸的效率.

主要方法:

通过表达两个编码子优化的细菌L-乳酸酸脱酶基因来改造S. stipitis.
利用强大的本地促进剂来驱动基因表达.
优化发酵条件,包括温度和pH值控制.

主要成果:

从葡萄糖中获得的乳酸位为7.42g/L (0.46g/g收益率),从西洛斯中获得的乳酸位为11.67g/L (0.58g/g收益率).
在74小时后,从50g/L的西洛斯中达到19.27g/L (0.52g/g收益率) 的最大标位.
优化发酵温度 (32°C) 和中和剂分别提高了30%和25%的产量.
改造品种只在葡萄糖上制造乙醇,而不是在糖上制造乙醇,与野生品种不同.

结论:

工程化S. stipitis是一种可行的宿主,可以从纤维素糖中产生微生物乳酸盐.
优化的发酵条件显著提高了乳酸产量和生产力.
乳酸的选择性生产比以醇的选择性生产具有生物技术上的优势.

关键词:

在L-乳酸酸脱酶.这种植物是 Scheffersomyces stipitis.代码子优化的基因表达.代谢工程是代谢工程.微生物的乳酸生产.非传统的酵母是非传统的酵母氧化糖发酵氧化糖发酵酵母细胞工厂是一个酵母细胞工厂.

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