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Sec61s和Sec62/Sec63基因通过调节Locusta migratoria的肠道和皮层发育,对生存至关重要

Xiaojian Liu^1,2, Mingzhu Ji^1,2, Jianzhen Zhang^1,2

¹Institute of Applied Biology, Shanxi University, Taiyuan 030006, China.

|June 25, 2025

概括

Sec61和Sec62/Sec63基因对于虫的肠道和皮质的发育至关重要. 沉默这些基因会导致严重的肠缩和变缺陷,突出显示它们作为杀虫剂目标的潜力.

科学领域:

分子生物学分子生物学
昆虫生理学昆虫生理学
害虫控制害虫控制害虫控制害虫控制害虫控制害虫控制

背景情况:

在真核生物中,Sec61通道促进了蛋白质穿过内细胞网膜的运输.
翻译后的蛋白质运输还需要Sec62/Sec63复合体.
这些Sec基因的功能在昆虫,特别是虫中基本未被探索.

研究的目的:

在迁徙虫Locusta migratoria*中识别和描述Sec61,Sec62和Sec63基因.
为了研究这些基因在各种虫组织中的表达模式.
阐明这些Sec基因在虫发育和生存中的功能作用.

主要方法:

在Locusta migratoria*中识别了五个Sec基因 (*Sec61α*, *Sec61β*, *Sec61γ*, *Sec62*, *Sec63*).
定量逆转录聚合酶连锁反应 (RT-qPCR) 用于基因表达分析.
基因淘汰的RNA干扰 (RNAi),然后进行死亡率评估,组织学分析 (血素-氨酸染色) 和传输电子显微镜 (TEM).

主要成果:

在多种组织中表达了sec基因,在体中表达高.
对*LmSec61α*和*LmSec61γ*的抑制导致了停止食,100%的死亡率,肠缩以及中肠细胞和微小的严重损伤.
*LmSec62*和*LmSec63*的淘汰导致了变过程中的高死亡率,而*LmSec63*的沉默抑制了旧皮层的降解和新皮层的形成,并影响了微尖结构.

结论:

Sec61s和Sec62/Sec63基因对于虫的正常肠道和皮质发育至关重要.
这些基因的干扰会导致严重的发育失败,包括肠缩和变缺陷.
这些Sec基因代表了开发新的虫控制策略的有希望的分子标.

关键词:

龙迁徙者 (英语:Locusta migratoria) 是一种迁徙的动物.这是一种RNA干扰.在Sec61频道上.第62节/第63节复合体皮肤小部分的皮肤.我们的肠道是肠道.

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