Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.3K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

AT-HOME ASSESSMENT OF USABILITY AND DATA COLLECTION FROM A WEARABLE BIOELECTRONIC MONITORING DEVICE.

Proceedings of the ... Design of Medical Devices Conference. Design of Medical Devices Conference·2026
Same author

Analysis of Fall and Jump Behaviors in Freely Moving <i>Drosophila melanogaster</i> Using 58 fps Video.

Insects·2026
Same author

Regulatory T-cell based therapies for acute lung injury: a systematic review and meta-analysis.

American journal of respiratory cell and molecular biology·2026
Same author

Technology in Total Hip Arthroplasty: Recent Advancements.

Orthopedic reviews·2026
Same author

A multi-omic atlas in the African turquoise killifish reveals increased glucocorticoid signaling as a hallmark of brain aging.

bioRxiv : the preprint server for biology·2026
Same author

Including pregnant and breastfeeding women in clinical trials.

BMJ (Clinical research ed.)·2026

相关实验视频

Updated: Sep 18, 2025

Studying Membrane Protein Trafficking in Drosophila Photoreceptor Cells Using eGFP-Tagged Proteins
10:20

Studying Membrane Protein Trafficking in Drosophila Photoreceptor Cells Using eGFP-Tagged Proteins

Published on: January 21, 2022

2.9K

在自由移动的中,组织特异性光蛋白的周转率.

Katherine S Bell1, Sebastian Ko1, Sam Ali1

  • 1Molecular and Computational Biology Section, Department of Biological Sciences, University of Southern California, Los Angeles, CA 90089, USA.

Insects
|June 25, 2025
PubMed
概括

研究人员在果中开发了一种体内测试,以测量蛋白质的循环. 这种方法揭示了蛋白质降解率因蛋白质类型,组织和年龄而异,为蛋白质动力学提供了新的见解.

关键词:
这种植物是Drosophila.在 DsRED 中使用.美国GFP的GFP是GFP.博尔特佐米布 (Bortezomib) 是一种药物.循环赫西米德的使用方法降解降解降解降解降解.在活体中,活体中蛋白质酶体蛋白质组是什么公司营业额 营业额视频 视频 视频 视频 视频

更多相关视频

Measuring Protein Stability in Living Zebrafish Embryos Using Fluorescence Decay After Photoconversion FDAP
09:45

Measuring Protein Stability in Living Zebrafish Embryos Using Fluorescence Decay After Photoconversion FDAP

Published on: January 28, 2015

11.4K
Author Spotlight: Unveiling the Dynamics of Mechanical and Biochemical Signals in Animal Morphogenesis Research
07:15

Author Spotlight: Unveiling the Dynamics of Mechanical and Biochemical Signals in Animal Morphogenesis Research

Published on: January 19, 2024

1.2K

相关实验视频

Last Updated: Sep 18, 2025

Studying Membrane Protein Trafficking in Drosophila Photoreceptor Cells Using eGFP-Tagged Proteins
10:20

Studying Membrane Protein Trafficking in Drosophila Photoreceptor Cells Using eGFP-Tagged Proteins

Published on: January 21, 2022

2.9K
Measuring Protein Stability in Living Zebrafish Embryos Using Fluorescence Decay After Photoconversion FDAP
09:45

Measuring Protein Stability in Living Zebrafish Embryos Using Fluorescence Decay After Photoconversion FDAP

Published on: January 28, 2015

11.4K
Author Spotlight: Unveiling the Dynamics of Mechanical and Biochemical Signals in Animal Morphogenesis Research
07:15

Author Spotlight: Unveiling the Dynamics of Mechanical and Biochemical Signals in Animal Morphogenesis Research

Published on: January 19, 2024

1.2K

科学领域:

  • * 分子生物学 * 分子生物学
  • * 遗传学 在遗传学方面
  • * 生物化学 * 生物化学

背景情况:

  • * 蛋白质循环对于细胞功能至关重要,并且受到严格监管.
  • *现有的研究蛋白质降解的方法在体内有限.
  • *需要开发新的测定方法来理解蛋白质动态.

研究的目的:

  • * 建立一个强大的体内测试方法来测量成年Drosophila melanogaster中的蛋白质循环.
  • * 在体内确定各种光蛋白的半衰期.
  • * 调查影响蛋白质降解的因素,包括抑制剂,蛋白质类型,组织,年龄和性别.

主要方法:

  • *产生条件转基因Drosophila melanogaster表达光蛋白的基因.
  • * 在体内测量光蛋白质随时间的衰变,使用视频跟踪.
  • * 基于光衰变的蛋白质半衰期计算.
  • * 应用蛋白酶和蛋白质合成抑制剂来评估降解途径.
  • *分析不同组织,性别和年龄的蛋白质半衰期.

主要成果:

  • * 蛋白质酶抑制剂博特佐米布增加了EGFP的半衰期,证实了蛋白质酶酶的降解.
  • * 循环赫西米德降低了EGFP积累,但没有改变半衰期,这表明合成减少.
  • * 光蛋白的半衰期差异很大 (例如,DSRED > eGFP > mCherry).
  • *观察到半衰期的组织特异性差异 (例如,肌肉中更高的EGFP半衰期).
  • *对DSRED检测到半衰期的年龄相关变化,但没有发现eGFP.

结论:

  • * 开发的体内测定是一种有前途的工具,用于研究Drosophila中的蛋白质循环.
  • * 蛋白质降解受蛋白质内在特性,细胞局部和生理状态的影响.
  • * 这种测定有助于研究小分子对蛋白质降解途径的影响.