丝虫幼虫性腺体在对二暴露的反应中发生的基因病理和转录性变化
Tao Li1, Changxiong Hu1, Zenghu Liu1
1Sericulture and Apiculture Research Institute, Yunnan Academy of Agricultural Sciences, Mengzi 661101, China.
Insects
|June 25, 2025
概括
费纳皮尔杀虫剂暴露会通过影响淋巴体组织学和基因表达来破坏丝虫的繁殖发育. 这项研究揭示了涉及排毒和激素调节的分子途径,这对于了解杀虫剂风险至关重要.
科学领域:
- 昆虫学 昆虫学是一门学科.
- 毒理学 毒理学 毒理学
- 分子生物学分子生物学
背景情况:
- хлорфенапир 是一种广泛的杀虫剂,已知对非目标昆虫有影响.
- 它对丝虫 (Bombyx mori L.) 生殖发育的特定影响尚未完全理解.
- 研究这些影响对于评估生态风险和制定可持续的害虫管理策略至关重要.
研究的目的:
- 为了研究芬暴露后丝虫性腺体的组织病理学和转录性变化.
- 为了确定关键的分子通路和基因,涉及到Bombyx mori.在烯酸的反应.
- 提供有关芬对昆虫生殖健康影响的机制的见解.
主要方法:
- 对暴露于芬的丝虫幼虫的卵巢和丸进行组织病理学检查.
- 转录组分析 (RNA测序) 用于在暴露的生殖腺中识别差异表达基因 (DEG).
- 路径丰富分析,蛋白质-蛋白质相互作用 (PPI) 网络构建和定量PCR (qPCR) 验证.
主要成果:
- 组织病理学显示淋巴体发育延迟,卵巢内卵子/卵子细胞减少,丸中精子细胞减少.
- 转录组分析显示,DEGs富含"药物代谢-P450细胞染色体"",昆虫激素生物合成"和"核糖体"途径.
- 确定了高调节的解毒基因 (CYPs,GSTs) 和激素调节剂 (JHAMT,JHEH),以及改变的转录因子和枢纽基因 (EcR,Kr-h1).
结论:
- 二在组织和分子层面上显著损害了丝虫的繁殖发育.
- 丝虫激活了排毒和激素调节的途径,作为对二暴露的反应.
- 调查结果为二的生态风险评估提供了关键数据,并为农业和养殖中可持续的杀虫剂使用提供了信息.
相关概念视频
01:39Mutations
Overview
01:31Translation
Lesson: Translation
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
01:32RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
02:28Leaky Scanning
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R stands for...
02:32Exon Recombination
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Exon shuffling follows “splice frame rules.” Each exon has three reading...
01:31Translation
Lesson: Translation
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life