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从人类收集的Ixodes ricinus中分子检测和传播病原体的表征

Marina Žekić¹, Tjaša Cerar Kišek², Vesna Cvitković-Špik³

¹Scientific Veterinary Institute "Novi Sad", 21113 Novi Sad, Serbia.

Pathogens (Basel, Switzerland)

|June 25, 2025

概括

在塞尔维亚对的分子分析揭示了Borrelia burgdorferi sensu lato (s.l.) 的存在. 作为最常见的传播病原体. 这项研究强调了需要持续监测的必要性,以有效管理传播疾病.

科学领域:

兽医昆虫学兽医昆虫学
分子微生物学分子微生物学
公共卫生昆虫学昆虫学

背景情况:

是引起人类和动物疾病的病原体的重要载体.
传播疾病 (TBD) 构成了持续的威胁,需要研究有效的公共卫生战略.
了解传播病原体 (TBPs) 的流行率和多样性对于预防疾病至关重要.

研究的目的:

为了研究在塞尔维亚北部从人类身上收集的 *Ixodes ricinus* 中传播的关键病原体的分子特征.
为了确定 *Borrelia burgdorferi* sensu lato (s.l. burgdorferi* sensu lato) 的流行情况 ), *阿纳等离子体细胞* 和 *燃烧性菌*.
评估检测到的 *Borrelia* 种类的遗传多样性.

主要方法:

在2019年至2024年期间,从人类宿主中收集和检查Ixodes ricinus.
使用PCR或其他核酸放大技术进行分子分析,以检测和识别特定的传播病原体.
对*Borrelia*物种进行基因定型以确定遗传多样性.

主要成果:

*Borrelia burgdorferi* s.l.l. 是一个植物. 是最常见的病原体,在20.45%的中发现.
分子鉴定发现了两种基因: *Borrelia afzelii* 和 *Borrelia burgdorferi* sensu stricto (s.s.),表明遗传多样性.
在1.62%的虫中检测到*Anaplasma phagocytophilum*;没有检测到*Coxiella burnetii*.

结论:

这项研究强调了对种群的持续分子监测的重要性,特别是与人类接触的种群,以监测TBD风险.
这些发现有助于了解塞尔维亚的传播疾病的流行病学,塞尔维亚是莱姆病的流行地区.
数据支持实施有针对性的公共卫生干预措施,并根据病原体流行率和多样性改进疾病管理策略.

关键词:

亚纳等离子体的细胞菌体.波雷利亚 (Borrelia) 公司尾虫燃烧虫 (Coxiella burnetii) 已经被发现.塞尔维亚塞尔维亚塞尔维亚塞尔维亚传播疾病传播疾病

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