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人类乳头瘤病毒编码的microRNAs作为人类基因表达调节器在上状细胞癌:一个转录学研究研究

Daniel J García¹, Marco A Pulpillo-Berrocal¹, José L Ruiz^1,2

¹Institute of Parasitology and Biomedicine "López-Neyra" (IPBLN), Spanish National Research Council (CSIC), 18016 Granada, Spain.

|June 25, 2025

概括

来自人类乳头瘤病毒 (HPV) 的新型病毒微RNA (miRNA) 可能通过改变宿主基因表达来驱动门癌症的发展. 这些病毒性miRNA显示出作为 squamous cell carcinoma (ASCC) 的早期生物标志物和治疗点的潜力.

科学领域:

在瘤学瘤学.
病毒学病毒学
基因组学就是基因组学.

背景情况:

部平细胞癌 (ASCC) 是一种与瘤原性人类乳头瘤病毒 (HPV) 相关的罕见恶性瘤.
病毒非编码RNAs (ncRNAs) 在ASCC病变发生中的作用尚不清楚.
研究病毒的ncRNA为HPV驱动的瘤发生提供了洞察力.

研究的目的:

在门病变中识别和表征新型病毒微RNA (miRNA).
探索病毒miRNAs对ASCC.宿主基因表达的影响.
评估病毒miRNAs作为生物标志物和治疗点的潜力.

主要方法:

重新分析了来自70个内活检 (LGSIL,HGSIL,ASCC) 的RNA-Seq数据.
微生物组成的分类学评估.
使用vsRNAfinder预测新型病毒miRNAs,并使用TargetScan进行宿主目标分析.

主要成果:

在不同级别的病变中,alphapapillomavirus被丰富.
鉴定了90种新的病毒miRNA和177种抗相关miRNA-mRNA相互作用.
参与细胞循环,EMT,脂质代谢,免疫调节和病毒复制的目标基因.

结论:

由HPV衍生的miRNAs,包括来自低风险类型的miRNAs,可能有助于瘤转变.
病毒miRNAs是HPV相关门癌的潜在驱动因素.
病毒miRNAs显示作为早期生物标志物和ASCC的治疗点的承诺.

关键词:

门状细胞癌瘤在新的miRNAs中.人类乳头瘤病毒人类乳头瘤病毒在委员会上.状内皮层病变的发生.目标预测目标预测翻译学翻译学翻译学翻译学病毒的miRNAs是病毒的miRNAs.

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...