在自然条件下PCV2疫苗的有效性:使用PCR和病毒隔离的纵向研究
Eugene Mazimpaka1,2, Rissar Siringo Ringo1,2, Tasuku Hirooka2
1Graduate School of Medicine and Veterinary Medicine, University of Miyazaki, Miyazaki 889-2192, Japan.
Veterinary sciences
|June 25, 2025
概括
英格尔瓦克 (Ingelvac) 的CircoFLEX®疫苗降低了猪病毒2型 (PCV2) 基因负载,并防止了小猪的PCV2隔离. 评估PCV2疫苗的疗效应包括病毒隔离,PCR和抗体测试.
科学领域:
- 兽医病毒学 兽医病毒学
- 猪病研究研究 猪病研究
背景情况:
- 猪循环病毒2型 (PCV2) 通过猪循环病毒相关疾病 (PCVAD) 在猪中造成重大经济损失.
- 目前的抗PCV2疫苗的疗效有所变化,可能是由于同时感染和评估方法的局限性.
研究的目的:
- 在自然现场条件下评估英格尔瓦克CircoFLEX® PCV2疫苗的实际有效性.
- 为了比较PCV2检测率,基因负载和接种疫苗和未接种疫苗的小猪之间的抗体反应.
主要方法:
- 从接种疫苗和未接种疫苗的猪仔 (21-173天) 收集和分析了100个血清样本.
- 使用PCR进行PCV2检测和基因负载量化,抗体检测和病毒隔离.
- 评估与猪生殖和呼吸系统综合征病毒 (PRRSV) 的同时感染.
主要成果:
- 在91天后在接种疫苗的小猪中观察到显著较低的PCV2基因负载 (p = 0.0095).
- 没有从接种疫苗的小猪中分离PCV2,而在未接种疫苗的小猪中,这一比例为50%.
- 在91,145和173天接种疫苗的猪仔中显著降低抗PCV2抗体标位 (p < 0.0001).
- 在PCV2基因负载激增之前,在两组中都检测到了PRRSV联合感染,这表明在增强PCV2复制方面可能发挥作用.
结论:
- 在自然条件下,英格尔瓦克CircoFLEX®疫苗在降低PCV2基因负载和预防病毒病方面表现出有效性.
- 病毒隔离是评估PCV2疫苗疗效的关键方法,补充PCR和抗体标位评估.
- 与PRRSV的同时感染可能会影响PCV2病原和疫苗反应.
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