赛拉诺斯® 320 eNose 在诱导牛呼吸系统疾病模型中有效区分挑战前和挑战后的呼吸道样本
Conrad S Schelkopf1, Leslie F Weaver2, Michael D Apley2
1Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA.
电子鼻子 (eNose) 在早期发现牛呼吸道疾病 (BRD) 方面表现有前途. 鼻子扫描提供了比呼出的空气更高的准确性来诊断小牛的BRD.
科学领域:
- 兽医医学 兽医医学 兽医医学
- 动物卫生诊断 动物卫生诊断
- 生物传感器技术技术
背景情况:
- 牛呼吸道疾病 (BRD) 对牛的福利构成重大挑战,需要有效的抗菌药物管理.
- 早期发现BRD对于及时干预和改善群体管理至关重要.
- 需要基于现场的诊断工具来补充传统的实验室方法.
研究的目的:
- 评估电子鼻子 (eNose) 在霍尔斯坦小牛中早期检测诱导的牛呼吸系统疾病 (BRD) 的有效性.
- 为了比较eNose分析的诊断准确性,使用鼻与过期的空气样本进行分析.
- 评估eNose作为BRD的现场部署诊断技术的潜力.
主要方法:
- 在12只霍尔斯坦小牛中使用诱导的BRD模型,每天抽取鼻分泌物和呼出的空气样本.
- 在挑战前和挑战后期间,使用电子鼻子 (eNose) 分析了样本.
- 牛犊受到牛性疹病毒-1 (BHV-1) 和曼海米亚血解菌的挑战.
主要成果:
- 在挑战前的样本分类中,eNose实现了高准确性:鼻子拭子的93.5%,气息的96.8%.
- 在挑战后,eNose的精度为97.8%的鼻子拭子和72.5%的呼气.
- 样本类型之间的差异在6日和7日后的细菌挑战中最为明显.
结论:
- 电子鼻子 (eNose) 显示出作为牛呼吸道疾病 (BRD) 的现场诊断工具的巨大潜力.
- 由于更高的准确性,鼻被确定为基于eNose的BRD检测的最佳样本类型.
- 进一步开发可以提高过期空气分析对BRD诊断的实用性.
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