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卡瓦克罗尔通过ROS爆发诱导Aspergillus niger中的亡

Qingchao Gao^1,2,3,4, Zhiruo Feng^1,2,3,4, Zuli Wang⁵

¹Special Food Research Institute, Qingdao Agricultural University, Qingdao, 266109, People's Republic of China.

World journal of microbiology & biotechnology

|June 25, 2025

概括

卡瓦克罗尔触发了黑色阿斯伯菌中的活性氧物种 (ROS),导致细胞膜损伤和死亡. 这种抗真菌机制涉及调节NADPH氧化酶基因表达,为生物控制应用提供了潜力.

科学领域:

微生物学微生物学
生物化学生物化学
植物科学植物科学

背景情况:

卡瓦克罗尔是精油的关键成分,具有广泛的抗微生物特性.
它的抗真菌机制,特别是ROS诱导,需要在分子层面进一步阐明.

研究的目的:

为了研究由卡瓦克罗尔产生的ROS诱导的黑色阿斯伯菌的亡机制.
为了澄清参与卡尔瓦克罗尔抗真菌作用的分子途径.

主要方法:

诱导ROS爆发和过氧化积累由卡瓦克罗尔.
对细胞膜完整性,脂质过氧化和脂肪酸/甘油脂含量的分析.
用RT-qPCR来评估NADPH氧化酶子单元的基因表达 (NoxA,Cdc24,NoxD,NoxR).
使用ROS清除剂和NADPH氧化酶抑制剂进行验证.

主要成果:

卡瓦克罗尔诱导ROS爆发和细胞内H2O2积累在A. niger中,导致氧化应激.
观察到脂质过氧化,改变了膜脂肪酸和糖脂含量,破坏了膜完整性.
证实了NoxA,Cdc24,NoxD和NoxR基因表达的升级.
卡瓦克罗尔的抑制作用被ROS清除剂减少,并被NADPH氧化酶抑制剂阻断.

结论:

卡瓦克罗尔通过NADPH氧化酶途径触发ROS爆发来诱导A. niger细胞死亡.
这导致膜脂质过氧化,氧化还原失衡和不可逆转的细胞损伤.
这些发现为潜在的生物控制策略提供了对卡尔瓦克罗尔抗真菌活性的分子见解.

关键词:

黑色阿斯珀吉勒斯 (Aspergillus niger) 是一种抗真菌机制抗真菌机制卡尔瓦克罗尔 (Carvacrol) 是一种精油精油精油精油氧化压力是一种氧化压力.

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