高活性益生菌酸奶通过调节免疫反应和肠道微生物群来对抗小鼠中循环胺诱导的免疫抑制
Liyuan Yun1,2,3, Jinpeng Zhang1, Tiantian Zhao1
1College of Food Science and Bioengineering, Tianjin Agricultural University, Tianjin, 300392, China.
Probiotics and antimicrobial proteins
|June 25, 2025
概括
高活性益生菌酸奶通过改善肠道健康和激活关键免疫通路,在免疫抑制小鼠中增强了免疫功能. 较低剂量的益生菌对肠道微生物群的多样性和丰富性产生了更大的益处.
科学领域:
- 免疫学 免疫学 免疫学
- 微生物学 微生物学
- 胃肠病学 胃肠病学
背景情况:
- 益生菌调节免疫反应,保持肠道屏障的完整性.
- 环胺 (CTX) 诱导免疫抑制,影响免疫器官指数和肠道微生物群.
- 了解益生菌对CTX诱导的免疫抑制的影响对于免疫健康至关重要.
研究的目的:
- 为了研究高活性益生菌酸奶对循环胺诱导免疫抑制小鼠的免疫调节作用.
- 评估不同益生菌度对免疫器官指数,细胞因子生产和肠道结构的影响.
- 探索益生菌酸奶对肠道微生物群组成和TLR4/NF-κB信号通路的影响.
主要方法:
- 使用环胺 (CTX) 建立一种免疫抑制小鼠模型.
- 在14天内服用不同度的高活性益生菌酸奶 (LPY,MPY,HPY).
- 分析使用HE染色,ELISA,西部涂抹和16S rRNA测序.
主要成果:
- 高活性益生菌酸奶显著增加了免疫器官指数和促炎细胞因子 (IL-6,IL-12p70,TNF-α).
- 观察到肠道结构的改善和TLR4,p65和TRAF6的增强表达.
- 益生菌酸奶增加了肠道微生物群的多样性和丰富性,较低剂量 (LPY) 显示出更好的效果.
结论:
- 高活性益生菌酸奶通过增强肠道平衡来改善CTX诱导的免疫抑制.
- 益生菌酸奶激活TLR4/NF-κB信号通路,有助于改善免疫功能.
- 特定的益生菌度,特别是较低的剂量,可以优化肠道微生物群的调节,以获得免疫效益.
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