相关实验视频

Updated: Sep 18, 2025

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

反向工程神经元类型特定和类型直角拼接调节网络使用多种细胞转录组

Daniel F Moakley¹, Melissa Campbell¹, Miquel Anglada-Girotto²

¹Department of Systems Biology, Columbia University, New York, NY 10032, USA; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA; Center for Motor Neuron Biology and Disease, Columbia University, New York, NY 10032, USA.

|June 25, 2025

概括

这项研究映射了133种小鼠新皮层细胞类型的替代拼接 (AS) 的RNA结合蛋白 (RBP) 调节. 我们确定了关键的RBPs,如Elavl2,驱动神经元中的细胞类型特定的拼接模式.

科学领域:

神经科学是一个神经科学.
分子生物学分子生物学
计算生物学计算生物学

背景情况:

替代拼接 (AS) 产生各种基因异型,对神经元类型特定功能至关重要.
目前对RNA结合蛋白 (RBPs) 和AS的研究仅限于少数神经元类型,需要进行全面分析.

研究的目的:

在133种小鼠新皮层细胞类型中创建替代拼接监管格局的整体地图.
确定RNA结合蛋白 (RBPs) 和它们的细胞类型特异性活动,以控制替代拼接 (AS).

主要方法:

网络逆向工程被应用于来自单细胞RNA测序数据的伪球体转录组.
350个RBP的推断调节和它们的细胞类型特定活动.
通过实验室胚胎干细胞 (ESC) 分化系统验证了Elavl2在中间质突出 (MGE) 特定拼接中的作用.

主要成果:

为133种小鼠新皮层细胞类型生成了神经元类型特定的AS调控格局的全面地图.
推断了350个RBPs的规律和细胞类型特定活动.
埃拉夫l2被证实是GABAergic内部神经元中MGE特定拼接的关键RBP.
确定了一个对长短投射神经元特异的外显子和候选调节器模块.

结论:

这项研究为了解导致神经元分子多样性的拼接监管程序提供了宝贵的资源.
这些发现揭示了可能无法通过基因表达基因分类捕获的拼接调节.
已识别的RBPs和拼接模块为推动神经元异质性的机制提供了洞察力.

关键词:

科普:分子生物学分子生物学科普:神经科学是什么意思第122章没有了 RNA拼接规则的调节 RNA结合蛋白质是RNA结合的蛋白质.网络推断网络推断神经元亚型的神经元亚型

更多相关视频

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

相关概念视频

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

Chromatin Structure and RNA Splicing

Chromatin Structure and RNA Splicing

Pre-mRNA Processing: RNA Splicing

Pre-mRNA Processing: RNA Splicing

What is Gene Expression?

What is Gene Expression?

A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...