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3,4-胺芬作为一种有效的反应性MALDI矩阵,用于简单而灵敏地检测α-酸化合物

Mengzhen Wang¹, Ling Ling¹, Jie Wen¹

¹Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang Province, Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo 315211, China.

Analytical chemistry

|June 25, 2025

概括

一种新的矩阵辅助激光脱/电离飞行时间质谱法 (MALDI-TOF MS) 方法使用3,4-diaminobenzophenone (DABP) 进行对α-ketoacids的敏感检测. 这种方法可以在食品和药品等复杂样品中进行快速和选择性分析.

科学领域:

分析化学分析化学
生物化学生物化学
质谱测量质量谱测量

背景情况:

精确地确定α-酸对于疾病诊断和食品和药品的质量控制至关重要.
现有的α-酸分析方法可能是复杂的,耗时的,或缺乏特异性.

研究的目的:

开发一种简单,快速和同时的方法来确定复杂样品中的α-酸.
为了提高α-酸检测的灵敏度和选择性,使用矩阵辅助激光脱/电离飞行时间质谱法 (MALDI-TOF MS).

主要方法:

为马尔迪-托夫MS开发一种新型反应矩阵,即3,4-胺二芬 (DABP),用于马尔迪-托夫MS.
DABP与alpha-ketoacids的反应,形成意达衍生物,以改善电离.
优化MALDI-TOF MS方法的灵敏度,线性,准确性和可重现性.

主要成果:

使用DABP的拟议方法与常见的性矩阵相比,显著提高了电离效率.
达到了低检测极限 (0.8μM为α-甲酸,0.45μM为酸).
证明了出色的线性 (R2>0.999),高精度 (95.1-102.9%) 和良好的可重现性 (RSD:0.8-4.9%).

结论:

开发的基于DABP的MALDI-TOF MS方法提供了一种敏感,快速和选择性的方法来确定α-ketoacid.
该方法已成功应用于现实世界样本,包括补充剂,洋和酒.
这种技术显示出在复杂矩阵中对alpha-ketoacids进行常规分析的巨大潜力.

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