相关实验视频

Updated: Sep 18, 2025

Design and Use of Multiplexed Chemostat Arrays

Design and Use of Multiplexed Chemostat Arrays

Published on: February 23, 2013

祖先的[Fe-S]生物发生系统SMS具有集群组装和硫利用的独特机制

Macha Dussouchaud¹, Markel Martinez-Carranza², Pierre-Simon Garcia³

¹Department of Microbiology, Unit Stress Adaptation and Metabolism in Enterobacteria, Institut Pasteur, Université Paris Cité, UMR CNRS 6047, Paris, France.

|June 25, 2025

概括

古代的SMS系统对于生命早期[Fe-S]集群生物发生至关重要,它使用SmsC2B2组装和分发集群,即使在缺乏这些通路的现代细菌中也能发挥作用.

科学领域:

生物化学和分子生物学
天体生物学与生命的起源
生物能量学和新陈代谢学

背景情况:

[Fe-S]集群是众多细胞过程的重要辅助因子,起源于古代无氧环境.
最少的铁硫 (MIS) 和硫动员 (SMS) 系统是提出的祖先[Fe-S]生物发生路径,早于现代ISC,NIF和SUF机械.
超热友的考古生物Methanocaldococcus jannaschii拥有一个祖先的短信系统.

研究的目的:

从生物化学和结构上描述来自Methanocaldococcus jannaschii的祖先SMS系统.
阐明SMS系统中[Fe-S]集群组装和调节的机制.
评估SMS系统的功能保护和进化意义.

主要方法:

生物化学测定和SmsC2B2异质四胺的结构研究.
在SmsC氨酸残留物的位点定向突变发生.
在体外[Fe-S]集群转移实验.
在工程化大肠杆菌菌株中进行功能补充测试.

主要成果:

在[Fe-S]集群生物发生过程中,SmsC2B2复合体发挥作用,SmsC以相互排斥的方式结合ATP和集群.
在SmsC中的特定的氨酸残留物对[Fe-S]集群组装至关重要.
短信系统拯救了一个无氧的大肠杆菌辅助体,利用硫化物作为硫源.
结构和功能证据表明,大多数古生物中的SmsC蛋白质具有类似的集群组装站点.

结论:

短信系统是一种独特的[Fe-S]集群生物发生路径,在无氧 prokaryotes 中运行.
SmsC2B2采用了一个规范的机制来组装和分发 [4Fe-4S] 集群.
短信系统可能早于硫动员 (SUF) 系统,在大氧化事件后演变.

更多相关视频

Author Spotlight: Exploring Cytoskeletal Dynamics to Unveil Novel Antibiotics Through Innovative Cell-Based Assays

Author Spotlight: Exploring Cytoskeletal Dynamics to Unveil Novel Antibiotics Through Innovative Cell-Based Assays

Published on: April 26, 2024

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor

Published on: October 15, 2015

相关概念视频

Sulfur Assimilation

Sulfur Assimilation

Sulfur is an essential element in biological systems, contributing to synthesizing key biomolecules, including amino acids such as cysteine and methionine, and cofactors such as coenzyme A and biotin. Microorganisms primarily assimilate sulfur as sulfate (SO₄²⁻) from the environment, which must undergo a series of biochemical transformations before it can be incorporated into cellular components. As sulfate is highly oxidized, it must undergo assimilatory sulfate reduction to...

Cell Inclusions

Cell Inclusions

Prokaryotic cells possess a variety of inclusions that play crucial roles in nutrient storage, metabolic processes, and environmental adaptation. These structures enable bacteria to thrive under fluctuating environmental conditions by storing essential resources and optimizing their metabolic efficiency.Carbon Storage: Poly-β-Hydroxybutyric Acid and Glycogen GranulesBacteria frequently store excess carbon in specialized granules. Poly-β-hydroxybutyric acid (PHB) granules are lipid...

Anoxygenic Photosynthesis

Anoxygenic Photosynthesis

Anoxygenic photosynthesis is a phototrophic process that captures light energy to drive carbon fixation without producing molecular oxygen. Unlike oxygenic photosynthesis, which utilizes water as an electron donor and releases oxygen, anoxygenic phototrophs use alternative electron donors such as hydrogen sulfide (H₂S), elemental sulfur (S⁰), or thiosulfate (S₂O₃²⁻). This process is carried out by diverse groups of bacteria, including purple bacteria, green...

Biosynthesis in Bacteria

Biosynthesis in Bacteria

Biosynthesis in bacteria is a fundamental anabolic process that generates essential macromolecules, including proteins, nucleic acids, lipids, and polysaccharides. These macromolecules are critical for cellular growth, replication, and function. The process is tightly regulated and energetically linked to catabolic pathways to ensure optimal resource utilization.Biosynthetic pathways begin with precursor metabolites such as pyruvate, acetyl-CoA, and glucose-6-phosphate derived from glycolysis,...

Protein Complexes with Interchangeable Parts

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...

Microbial Nutrition

Microbial Nutrition

Organisms exhibit remarkable metabolic diversity, categorized based on how they acquire energy and carbon. These strategies enable survival in various ecological niches and are essential for maintaining energy flow and nutrient cycling within ecosystems.Energy and Carbon SourcesOrganisms are classified as phototrophs or chemotrophs based on energy acquisition. Phototrophs use light as their energy source, while chemotrophs rely on oxidizing chemical compounds. Further differentiation arises...