由AREB和MYB转录因子介导的协调色素修饰维持了Populus的干旱耐受性
Jinghui Gao1, Xiaoqian Wu1, Rui Zhai1
1State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China.
Plant physiology
|June 25, 2025
概括
植物使用转录记忆来耐受干旱. 这项研究揭示了PtrMYB161和PtrAREB1-2蛋白与酸转移酶复合体如何在表观遗传学上调节PtrNAC120基因表达,使Populus持续耐旱.
科学领域:
- 植物生物学 植物生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
背景情况:
- 持续的植物干旱耐受性涉及跨越压力周期的转录记忆.
- 这种记忆的基于染色体的机制尚未完全理解.
- 在Populus trichocarpa中过度表达PtrMYB161赋予了耐旱表型.
研究的目的:
- 阐明PtrMYB161对干旱耐受性的表观遗传机制.
- 为了研究PtrMYB161,PtrAREB1-2和氨酸转移酶复合体在调节干旱反应基因中的相互作用.
- 了解Populus.在持续干旱耐受性的协调表观遗传调节.
主要方法:
- 在Populus trichocarpa中过度表达和转基因的PtrMYB161.
- 染色体免疫沉 (ChIP) 测试检测蛋白质结合和基因素修饰.
- 使用定量PCR对基因表达的分析.
- 用于功能研究的原生质量测试.
主要成果:
- PtrMYB161直接与PtrNAC120促进体结合,并招募氨酸转移酶 (HAT) 复合体PtrGCN5-1-PtrADA2b-3.
- 这种复合物增强了H3K9,H3K14和H3K27的乙化,增加了RNA Pol II占用率和PtrNAC120表达.
- 干旱诱导的PtrAREB1-2也形成了一个HAT复合体来激活PtrNAC120,而PtrMYB161在严重压力下起到辅助作用.
- 功能损失和功能增益的实验证实了PtrMYB161和PtrAREB1-2.2的调节作用.
结论:
- 通过HAT复合体的协调表观遗传调节,涉及PtrMYB161和PtrAREB1-2,维持了Populus的干旱耐受性.
- PtrMYB161建立了一个干旱耐受性的表观遗传记忆.
- 在压力下,PtrAREB1-2系统激活了PtrMYB161介导的辅助调节.
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