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双相融合蛋白疫苗诱导了对SARS-CoV-2和金黄色葡萄球菌的保护性免疫力

Menghan Ma¹, Jintao Zou², Xiaoqiang Zeng³

¹School of Biological Engineering, Dalian Polytechnic University, Dalian, China; Laboratory of Advanced Biotechnology, Beijing Institute of Biotechnology, Beijing, China; Nanhu Laboratory, Jiaxing, China.

|June 25, 2025

概括

一种新的融合疫苗HRBD结合了SARS-CoV-2和S. aureus成分,以对抗共感染. 这种新型疫苗有效地中和了两种病原体,并防止了病毒和细菌的联合挑战.

科学领域:

免疫学免疫学免疫学
疫苗学疫苗学疫苗学
微生物学微生物学

背景情况:

病毒-细菌联合感染对全球健康构成重大挑战.
现有的疫苗对病毒和细菌病原体同时提供有限的保护.

研究的目的:

开发和评估一种新型的融合蛋白疫苗,HRBD,针对SARS-CoV-2和金黄色葡萄球菌.

主要方法:

构建了一个融合蛋白疫苗 (HRBD),结合了SARS-CoV-2受体结合域 (RBD) 和一个排毒的α-hemolysin突变体 (HlaH35A).
评估了小鼠的免疫反应 (IgG1,IL-4,IFN-γ) 和对SARS-CoV-2伪病毒和S. aureus血解活性的中和活性.
在HACE2转基因小鼠中评估了疫苗的疗效,这些小鼠受到SARS-CoV-2伪病毒和S. aureus的挑战.

主要成果:

HRBD诱导了针对SARS-CoV-2和S. aureus抗原的强大的IgG1主导的抗体反应.
该疫苗对SARS-CoV-2伪病毒 (NT50~10^3) 和S. aureus溶血活性 (NT50~10^2) 具有中和作用.
HRBD显示剂量节约的有效性和保护小鼠免受联合感染的挑战,促进平衡的Th1/Th2免疫反应.

结论:

HRBD是一种有前途的候选疫苗,用于预防由SARS-CoV-2和S. aureus引起的共感染.
融合蛋白方法为开发多病原体疫苗提供了一个潜在的策略.

关键词:

这是一种双等效疫苗.聚变蛋白质的融合蛋白质是如何形成的在RBD中,RBD是RBD.病毒与细菌的同时感染.α-Hemolysin是什么? α-Hemolysin是什么? α-Hemolysin是什么? α-Hemolysin是什么?

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