相关实验视频

Updated: Sep 18, 2025

Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes

Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes

Published on: June 3, 2018

抑制mitoNEET可以恢复线粒体的氧化还原稳态,并减弱肌纤维细胞分化

Byeong Geun Seok¹, Sangguk Lee¹, Su Hyeon Jin¹

¹Department of Biological Sciences, College of Natural Sciences, University of Ulsan, Ulsan, 44610, South Korea.

Free radical biology & medicine

|June 25, 2025

概括

在肺纤维化中,MitoNEET蛋白调节肌纤维细胞分化. 抑制mitoNEET可降低活性氧物种,并可能为异常性肺纤维化提供新的治疗策略.

科学领域:

生物化学生物化学
细胞生物学细胞生物学
肺部医学肺部医学

背景情况:

异形性肺纤维化 (IPF) 是一种渐进的肺部疾病,以痕和细胞外基质积累为特征.
分化的肌纤维细胞驱动纤维化,反应性氧物种和线粒体功能障碍与它们的发展有关.
米托尼特 (MitoNEET,CDGSH铁硫域含蛋白-1) 是一种调节铁恒温和活性氧物种的线粒体蛋白,但其在纤维化中的作用尚不清楚.

研究的目的:

研究 mitoNEET 在转化生长因子-β (TGF-β) 诱导的肌纤维细胞分化中的作用.
为了确定mitoNEET是否在肺纤维化背景下充当氧化还原调节剂.

主要方法:

用TGF-β治疗肺纤维细胞以诱导肌纤维细胞分化.
在TGF-β治疗后测量了MitoNEET表达 (mRNA和蛋白质).
评估了药理上的mitoNEET抑制和mitoNEET淘汰 (使用短毛RNA) 对肌纤维细胞分化和线粒体活性氧物种的影响.

主要成果:

TGF-β治疗显著增加了 mitoNEET mRNA 和肺纤维细胞中的蛋白质水平.
药理上抑制或击倒 mitoNEET 减弱的TGF-β诱导的肌纤维细胞分化.
这些效应与线粒体活性氧物种水平的降低有关.

结论:

米托尼特 (MitoNEET) 在通过氧化还原介导机制调节肌纤维细胞分化方面发挥着至关重要的作用.
在肺纤维化的进展中,MitoNEET 作为一个关键的氧化还原调节剂.
针对 mitoNEET 恢复氧化还原平衡,为异常性肺纤维化症提供了一个潜在的新疗法策略.

关键词:

纤维细胞分化的差异化异形性肺纤维化症异形性肺纤维化症线粒体的ROS是什么?有反应性氧物种的反应性氧物种.这就是 mitoNEETNEET 的意思.

更多相关视频

Isolation of Primary Myofibroblasts from Mouse and Human Colon Tissue

Isolation of Primary Myofibroblasts from Mouse and Human Colon Tissue

Published on: October 12, 2013

Author Spotlight: Optogenetic Inhibition of Rho1-Mediated Actomyosin Contractility Coupled with Measurement of Epithelial Tension in Drosophila Embryos

Author Spotlight: Optogenetic Inhibition of Rho1-Mediated Actomyosin Contractility Coupled with Measurement of Epithelial Tension in Drosophila Embryos

Published on: April 14, 2023

相关概念视频

Formation of Muscle Fibers from Myoblasts

Formation of Muscle Fibers from Myoblasts

De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...

Electron Transport Chain: Complex I and II

Electron Transport Chain: Complex I and II

The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...

Mitochondrial Membranes

Mitochondrial Membranes

A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...