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鉴定海帕福林作为一种新型抗病毒化合物,用于抗击登革热病毒

Shengchen Bai¹, Huiru Liang¹, Weihao Jiang²

¹Department of Pathogen Biology, School of Public Health, Southern Medical University, Guangzhou 510515, Guangdong, China.

Antiviral research

|June 25, 2025

概括

研究人员确定了低氨酸作为潜在的登革热病毒治疗药物. 这种天然化合物抑制病毒甲基转移酶活性,降低病毒复制的低毒性,为药物发现提供了新的途径.

科学领域:

病毒学病毒学
药物发现药物发现药物发现
生物化学生物化学

背景情况:

登革热病毒每年在全球感染大约3.90亿人.
目前没有针对登革热的特定抗病毒疗法得到批准.
登革热病毒 (DENV) NS5蛋白的甲基转移酶 (MTase) 域对病毒复制至关重要.

研究的目的:

确定针对登革热病毒的新型治疗剂.
作为DENV NS5蛋白MTase活性的潜在抑制剂,研究海帕福林.
为了建立作为抗病毒剂的海帕福林的结构-活性关系.

主要方法:

针对DENV NS5 MTase域的潜在连接体的化分子对接选.
在体外验证使用细胞热转移试验 (CETSA) 和表面等离子体共振成像 (SPRi).
在细胞培养模型 (BHK-21和Huh-7细胞) 中,酶分析以确定海帕福林对MTase活性的抑制和抗病毒功效.

主要成果:

希帕福林证明了与DENV NS5 MTase域的高亲和力结合 (DENV2的结合能为-6.657 kcal/mol,DENV3的结合能为-6.663 kcal/mol).
实验验证证证实了与2.19 × 10−9 M的解离常数 (KD) 的直接目标接触.
希帕福林抑制了MTase活性 (IC50=29.9μM) 并抑制了细胞培养中的病毒复制 (IC50=18.85μM在BHK-21,15.7μM在Huh-7) 具有较低的细胞毒性 (CC50>600μM).
在感染前或感染后给药时观察到抗病毒疗效,与MTase抑制相关.

结论:

希帕福林被确定为一种新型,一流的登革热病毒NS5甲基转移酶的天然抑制剂.
这项研究强调了结构导向药物发现方法在开发抗黄病毒药物的有效性.
希帕福林作为登革热治疗的潜在治疗候选药物显示出希望.

关键词:

登革热病毒感染者希帕福林 (Hypaphorine) 是一种表情.甲基转移酶的使用方法非结构蛋白 5 非结构蛋白

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