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Updated: Sep 18, 2025

Residue-specific Incorporation of Noncanonical Amino Acids into Model Proteins Using an Escherichia coli Cell-free Transcription-translation System
11:47

Residue-specific Incorporation of Noncanonical Amino Acids into Model Proteins Using an Escherichia coli Cell-free Transcription-translation System

Published on: August 1, 2016

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通过可编程的伪尿素编辑和解码来扩展RNA编码子

Jiangle Liu1,2,3, Xueqing Yan1, Hao Wu1,3

  • 1The National Key Laboratory of Gene Function Studies and Manipulation, School of Life Sciences, Peking University, Beijing, China.

Nature
|June 25, 2025
PubMed
概括

研究人员开发了一种新的RNA编码扩展 (RCE) 策略,使用伪尿素 (Ψ) 编码来精确地在哺乳动物细胞中结合非正规氨基酸 (ncAA). 这种方法增强了特异性,并为遗传字母扩展提供了新的途径.

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