相关实验视频

Updated: Sep 18, 2025

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Published on: March 7, 2018

综合转录组分析和组合机器学习构建了ccRCC的乙化同静态模型,并验证了关键基因GCNT4

Baohua Zhu¹, Ziyang Mo¹, Yi Bao²

¹Department of Urology, The First Affiliated Hospital, Naval Medical University, Second Military Medical University, Shanghai, China.

Cancer cell international

|June 25, 2025

概括

这项研究确定了清细胞细胞癌 (ccRCC) 中关键的乙化相关基因,并开发了一种预后模型. 基因GCNT4显示出作为治疗点的潜力,抑制瘤生长和迁移.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
生物信息学是一种生物信息学.

背景情况:

清细胞细胞癌 (ccRCC) 是一种普遍存在的尿路系统恶性瘤.
蛋白质乙化对于调节细胞过程和癌症途径至关重要.
这项研究通过乙化透视来研究ccRCC机制.

研究的目的:

探索与蛋白质乙化相关的ccRCC的潜在生物机制.
为了确定影响ccRCC预后的乙化调节基因.
构建一个ccRCC患者预测结果的预测模型.

主要方法:

利用来自TCGA,ICGC和GEO数据库的RNA-seq和单细胞RNA测序数据.
采用机器学习算法来分析与乙化相关的差异表达基因 (DEG) 并构建预后风险模型.
进行基因组丰富分析 (GSEA) 并评估免疫透相关性. 通过细胞实验验证了GCNT4的功能.

主要成果:

确定了84个与ccRCC预后相关的乙化调节关键基因.
一个LASSO+RSF模型准确地预测了患者的存活率,高风险组显示出更糟糕的结果.
高GCNT4表达与更好的预后相关,抑制癌细胞增殖和入侵,并可能调节O-GlcNAc修饰.

结论:

使用转录组分析和机器学习开发了一个ccRCC乙化稳态模型.
验证了GCNT4作为与改善生存相关的关键基因.
在调节O-GlcNAc修饰方面,GCNT4的作用为ccRCC提供了潜在的治疗策略.

关键词:

乙化乙化是一种清细胞脏细胞癌.在 GCNT4 中.机器学习是机器学习.预测预后预测预测

更多相关视频

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics

Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics

Published on: June 17, 2012

相关概念视频

Combinatorial Gene Control

Combinatorial Gene Control

Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...

Constitutive and Regulated Gene Expression

Constitutive and Regulated Gene Expression

Gene expression in prokaryotes is governed by constitutive and regulated systems, allowing cells to balance the production of essential proteins with adaptive responses to environmental changes.Constitutive Gene ExpressionConstitutive, or housekeeping, genes are continuously expressed as they encode proteins vital for fundamental cellular processes. These include enzymes for glycolysis, ribosomal components for protein synthesis, and proteins involved in DNA replication. Their constant...

Cell Specific Gene Expression

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...