综合转录组和代谢组分析揭示了在不同碳酸盐应力下,大米种植根中复杂的氧化损伤机制
Yang Cao1, Fei Hao1, Jingpeng Li2,3
1College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, China.
Antioxidants (Basel, Switzerland)
|June 26, 2025
概括
碳酸 (Na2CO3) 的压力严重抑制了大米的生长,比碳酸 (NaHCO3) 的压力更严重. Na2CO3导致更大的根损伤,氧化应激和分子变化,影响苗中的辅酶通路和抗氧化剂系统.
科学领域:
- 农业科学 农业科学
- 植物生理学 植物生理学
- 分子生物学分子生物学
背景情况:
- 性压力 (AS) 显著影响到大米产量.
- 碳酸 (Na2CO3) 和碳酸 (NaHCO3) 对大米的差异性影响尚不清楚.
研究的目的:
- 研究Na2CO3和NaHCO3对苗生长,根健康,生理和分子反应的不同影响.
- 阐明大米对不同性成分的耐受性背后的机制.
主要方法:
- 苗经过对同等度的Na2CO3和NaHCO3.3离子进行了试验.
- 评估了生长参数,根损伤和生理指标 (ROS,MDA).
- 利用转录组和代谢组分析来确定分子变化.
主要成果:
- Na2CO3和NaHCO3都抑制了米的生长,Na2CO3显示出更明显的效果.
- Na2CO3导致更严重的根细胞膜损伤,并减少了根的活力.
- Na2CO3显著增加了活性氧物种 (ROS) 和甲 (MDA),表明氧化损伤更大.
- 转录组和代谢组分析显示,在Na2CO3压力下,更差异地表达的基因和代谢物.
- 在Na2CO3压力下丰富的途径包括奥素,甲酸盐,黄酸盐和谷氨代谢.
结论:
- 与NaHCO3.3相比,Na2CO3压力会在苗中引起更严重的氧化损伤和生理干扰.
- Na2CO3似乎干扰了辅酶信号传递,并压倒了内源性抗氧化剂系统.
- 这些发现为改善盐-性土壤中的盐耐受性提供了基础.
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